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Keratin Basic antibody

Reactivity: Human, Rat, Mouse, Rabbit, Cow, Chicken, Monkey, Dog WB, IF, FACS, IHC (p) Host: Mouse Monoclonal AE3 unconjugated
Catalog No. ABIN3023995
  • Target See all Keratin Basic Antibodies
    Keratin Basic
    Reactivity
    • 20
    • 11
    • 8
    • 6
    • 6
    • 5
    • 3
    • 2
    • 1
    • 1
    • 1
    Human, Rat, Mouse, Rabbit, Cow, Chicken, Monkey, Dog
    Host
    • 12
    • 9
    Mouse
    Clonality
    • 21
    Monoclonal
    Conjugate
    • 13
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Un-conjugated
    Application
    • 19
    • 17
    • 15
    • 13
    • 8
    • 4
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purification
    Protein G purified
    Immunogen
    Solubilized cytokeratin extract from human stratum corneum was used as the immunogen.
    Clone
    AE3
    Isotype
    IgG1
    Top Product
    Discover our top product Primary Antibody
  • Application Notes
    Differences in protocols and secondaries may cause the Basic Cytokeratin antibody to need titering for optimal performance.

    1. FFPE staining requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    1 mg/mL in 1X PBS, BSA free, sodium azide free
    Preservative
    Azide free
    Storage
    4 °C,-20 °C
    Storage Comment
    Store the Basic Cytokeratin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • Target
    Keratin Basic
    Alternative Name
    Basic Cytokeratin (Keratin Basic Products)
    Background
    Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. This antibody recognizes basic (Type II or HMW) cytokeratins, which include 67  kDa (CK1), 64  kDa (CK3), 59  kDa (CK4), 58  kDa (CK5), 56  kDa (CK6), 52  kDa (CK8). Many studies have shown the usefulness of keratin markers in cancer research and tumor diagnosis. The basic family clone AE3 is commonly used in combination with clone AE1 as a pan cytokeratin antibody acidic + basic cocktail (< a href=../pan-cytokeratin-antibody-cocktail-ae1-ae3-v2330>Cat No V2330).
    Gene ID
    3848
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