Nuclear Marker antibody (PE)

Catalog No. ABIN3023920

Product Details anti-Nuclear Marker Antibody (PE)

Target: Nuclear Marker

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nuclear Marker antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

No Cross-Reactivity: Mouse (Murine), Rat (Rattus), Chicken

Characteristics: This Nuclear marker antibody is part of a panel of reagents which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone 235-1 antibody specifically detects an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. The human nuclear antigen antibody can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Purity: Protein G affinity chromatography

Immunogen: Nuclei of human myeloid leukemia biopsy cells were used as the immunogen for this Nuclear marker antibody.

Clone: 235-1

Isotype: IgG1 kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Nuclear marker antibody to be titered up or down for optimal performance.\. Flow Cytometry : 5 μL/test/million cells or 5 μL/test/100 μL of whole blood,Immunofluorescence : 1:50-1:100 for 30 minutes at RT

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: 500 μL at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the human Nuclear antigen antibody at 2-8°C. Conjugate is light sensitive, store in the dark.

Target Details for Nuclear Marker

Target: Nuclear Marker

Abstract: Nuclear Marker Products