Nuclear Antigen antibody

Catalog No. ABIN3023918

Product Details

Target: Nuclear Antigen

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

No Cross-Reactivity: Mouse (Murine), Rat (Rattus), Chicken

Characteristics: This nuclear antigen antibody is part of a panel of reagents which recognizes subcellular organelles or compartments. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone 235-1 antibody specifically detects an antigen associated with the human nuclei. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. The nuclear antigen antibody can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Purification: Protein G affinity chromatography

Immunogen: Nuclei of human myeloid leukemia biopsy cells were used as the immunogen for this Nuclear antigen antibody.

Clone: 235-1

Isotype: IgG1 kappa

Application Details

Application Notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Nuclear antigen antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate Buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. IHC (Frozen): 0.5-1 μg/mL for 30 minutes at RT (1),Immunocytochemistry (Acetone-fixed): 0.25-0.5 μg/mL for 30 min at RT,FACS: 0.5-1 μg/10e6 cells,IF: 0.5-1 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the Nuclear Antigen antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details

Target: Nuclear Antigen

Background: This nuclear antigen antibody is part of a panel of reagents which recognizes subcellular organelles or compartments. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone 235-1 antibody specifically detects an antigen associated with the human nuclei. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. The nuclear antigen antibody can also be used with paraformaldehyde fixed frozen tissue or cell preparations.