Hepatocyte Specific Antigen antibody

Catalog No. ABIN3023750

Product Details anti-Hepatocyte Specific Antigen Antibody

Target: Hepatocyte Specific Antigen (HepPar-1)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Hepatocyte Specific Antigen antibody is un-conjugated

Application: Immunofluorescence (IF), Immunocytochemistry (ICC)

Characteristics: mAb HSA133 stains human liver canaliculi and a subset of hepatocellular carcinomas. In frozen sections, it stains liver canaliculi strongly and may be used as a marker of this hepatic substructure. Cell preparations of hepatocellular carcinoma biopsies and cell lines are found to bind this mAb on the cell surface. HSA133 strongly stains liver canaliculi and hepatic carcinoma cells using frozen sections or paraformaldehyde fixed cell preparations.

Purification: Protein G affinity chromatography

Immunogen: The human hepatocellular carcinoma cell line SK-H1A9-2 was used as the immunogen for this Hepatocyte Specific Antigen antibody.

Clone: HSA133

Isotype: IgG2b kappa

Application Details

Application Notes: The optimal dilution of the Hepatocyte Specific Antigen antibody for each application should be determined by the researcher.\. Immunofluorescence: 0.5-1 μg/mL,Immunocytochemistry (Acetone-fixed cells): 0.5-1 μg/mL for 30 minutes at RT

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Preservative: Azide free

Storage: 4 °C,-20 °C

Storage Comment: Store the Hepatocyte Specific Antigen antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Target Details for Hepatocyte Specific Antigen

Target: Hepatocyte Specific Antigen (HepPar-1)

Alternative Name: Hepatocyte Specific Antigen (HepPar-1 Products)

Background: MAb HSA133 stains human liver canaliculi and a subset of hepatocellular carcinomas. In frozen sections, it stains liver canaliculi strongly and may be used as a marker of this hepatic substructure. Cell preparations of hepatocellular carcinoma biopsies and cell lines are found to bind this mAb on the cell surface. HSA133 strongly stains liver canaliculi and hepatic carcinoma cells using frozen sections or paraformaldehyde fixed cell preparations.