Histone 3 antibody (H3K4me3)

Catalog No. ABIN3023254

Product Details anti-Histone 3 Antibody

Target: Histone 3 (H3)

Binding Specificity: H3K4me3

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Histone 3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP), Dot Blot (DB), ChIP DNA-Sequencing (ChIP-seq), Cleavage Under Targets and Release Using Nuclease (CUT&RUN), Cleavage Under Targets and Tagmentation (CUT&Tag)

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Methylated Antibodies

Purification: Affinity purification

Immunogen: A synthetic methylated peptide corresponding to residues surrounding K4 of human histone H3

Isotype: IgG

Application Details

Application Notes: DB,1:500 - 1:2000,WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200,IP,1:50 - 1:200,ChIP,1:20 - 1:100,ChIP-seq,1:20 - 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-Histone 3 antibody (ABIN3023254)

Janssens, Wu, Sarthy, Meers, Myers, Olson, Ahmad, Henikoff: "Automated in situ chromatin profiling efficiently resolves cell types and gene regulatory programs." in: Epigenetics & chromatin, Vol. 11, Issue 1, pp. 74, (2019) (PubMed).

Sun, Ermann, Niu, Yan, Yang, Shi, Zou: "Histone demethylase LSD1 regulates bone mass by controlling WNT7B and BMP2 signaling in osteoblasts." in: Bone research, Vol. 6, pp. 14, (2018) (PubMed).

Yin, Jia, Miron, Long, Xu, Wei, Wu, Zhang, Li: "Setd7 and its contribution to Boron-induced bone regeneration in Boron-mesoporous bioactive glass scaffolds." in: Acta biomaterialia, Vol. 73, pp. 522-530, (2018) (PubMed).

Liu, Wang, Gao, Liu, Liu: "iTRAQ-Based Proteomic Analysis of Neonatal Kidney from Offspring of Protein Restricted Rats Reveals Abnormalities in Intraflagellar Transport Proteins." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 44, Issue 1, pp. 185-199, (2018) (PubMed).

Yu, Liu, Liu, Wang, Liu, Miao, Du, Yang: "Ascorbic acid induces global epigenetic reprogramming to promote meiotic maturation and developmental competence of porcine oocytes." in: Scientific reports, Vol. 8, Issue 1, pp. 6132, (2018) (PubMed).

Target Details for Histone 3

Target: Histone 3 (H3)

Alternative Name: Histone H3 (H3 Products)

Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails, instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.,H3.4,H3/g,H3FT,H3t,HIST3H3,Histone H3,HIST1H3A,Signal Transduction,MAPK-Erk Signaling Pathway,MAPK-P38 Signaling Pathway,Epigenetics & Nuclear Signaling,Epigenetic Modifications,Methylation,Histone H3

Molecular Weight: 15 kDa

Gene ID: 8290

UniProt: Q16695