LIG4 antibody (AA 612-911)

Catalog No. ABIN3022475

Product Details anti-LIG4 Antibody

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Binding Specificity: AA 612-911

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LIG4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Sequence: SKHLYIGGDD EPQEKKRKAA PKMKKVIGII EHLKAPNLTN VNKISNIFED VEFCVMSGTD SQPKPDLENR IAEFGGYIVQ NPGPDTYCVI AGSENIRVKN IILSNKHDVV KPAWLLECFK TKSFVPWQPR FMIHMCPSTK EHFAREYDCY GDSYFIDTDL NQLKEVFSGI KNSNEQTPEE MASLIADLEY RYSWDCSPLS MFRRHTVYLD SYAVINDLST KNEGTRLAIK ALELRFHGAK VVSCLAEGVS HVIIGEDHSR VADFKAFRRT FKRKFKILKE SWVTDSIDKC ELQEENQYLI

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 612-911 of human DNA Ligase IV (NP_002303.2).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-LIG4 antibody (ABIN3022475)

Zhang, Chang, Sun, Chen, Yang, Tang, Jing, Kang, He, Wu, Wei, Wang, Xu, Zhu, Shen, Zeng, Wang, Liu, Zhang, Mao, Jiang, Sun: "Histone H3K27 methylation modulates the dynamics of FANCD2 on chromatin to facilitate NHEJ and genome stability." in: Journal of cell science, Vol. 131, Issue 12, (2018) (PubMed).

Li, Zhang, Chen, Guo, Zhang, Tang, Xu, Zhang, Tao, Wang, Jiang, Sun, Mao: "Impaired DNA double-strand break repair contributes to the age-associated rise of genomic instability in humans." in: Cell death and differentiation, Vol. 23, Issue 11, pp. 1765-1777, (2017) (PubMed).

Target Details for LIG4

Target: LIG4 (Ligase IV, DNA, ATP-Dependent (LIG4))

Alternative Name: LIG4 (LIG4 Products)

Background: The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.,LIG4,LIG4S,Epigenetics & Nuclear Signaling,Cell Biology & Developmental Biology,Apoptosis,LIG4

Molecular Weight: 103 kDa

Gene ID: 3981

UniProt: P49917

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response