Cyclin D1 antibody (C-Term)

Catalog No. ABIN3021887

Product Details anti-Cyclin D1 Antibody

Target: Cyclin D1 (CCND1)

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)

Sequence: PSMVAAGSVV AAVQGLNLRS PNNFLSYYRL TRFLSRVIKC DPDCLRACQE QIEALLESSL RQAQQNMDPK AAEEEEEEEE EVDLACTPTD VRDVDI

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: A synthetic peptide corresponding to a sequence within amino acids 200 to the C-terminus of human Cyclin D1 (NP_444284.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IF,1:50 - 1:200,IP,1:50 - 1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-Cyclin D1 antibody (ABIN3021887)

Wu, Xiong, Li, Yang, Ge et al.: "Integrated Proteomic and Transcriptomic Analysis Reveals Long Noncoding RNA HOX Transcript Antisense Intergenic RNA (HOTAIR) Promotes Hepatocellular Carcinoma Cell Proliferation by Regulating Opioid ..." in: Molecular & cellular proteomics : MCP, Vol. 17, Issue 1, pp. 146-159, (2019) (PubMed).

Deng, Wang, Zhao, Zhang, Chen, Zhao, Liu, Sun, Zhang, Ye, Du: "MICAL1 facilitates breast cancer cell proliferation via ROS-sensitive ERK/cyclin D pathway." in: Journal of cellular and molecular medicine, Vol. 22, Issue 6, pp. 3108-3118, (2018) (PubMed).

Wang, Huang, Zhang, Hou, Liu, Chen, Zhu, Zhang: "Toosendanin suppresses oncogenic phenotypes of human gastric carcinoma SGC‑7901 cells partly via miR‑200a‑mediated downregulation of β-catenin pathway." in: International journal of oncology, Vol. 51, Issue 5, pp. 1563-1573, (2018) (PubMed).

Li, Kang, Yin, Li, Chen, Li, Zhang, Qiu: "Ginsenoside Rh2 inhibits human A172 glioma cell proliferation and induces cell cycle arrest status via modulating Akt signaling pathway." in: Molecular medicine reports, Vol. 17, Issue 2, pp. 3062-3068, (2018) (PubMed).

Li, Lv, He, Wang, Zhang, Lu, Ren, Wang, Zhu, Ding, Liao, Ding, Liang: "The SOX17/miR-371-5p/SOX2 axis inhibits EMT, stem cell properties and metastasis in colorectal cancer." in: Oncotarget, Vol. 6, Issue 11, pp. 9099-112, (2016) (PubMed).

Gao, Wang, Zhao, Chen, Xie: "MicroRNA-204-5p-Mediated Regulation of SIRT1 Contributes to the Delay of Epithelial Cell Cycle Traversal in Diabetic Corneas." in: Investigative ophthalmology & visual science, Vol. 56, Issue 3, pp. 1493-504, (2015) (PubMed).

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: CCND1 (CCND1 Products)

Background: The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis.,BCL1,D11S287E,PRAD1,U21B31,CCND1,Cyclin D1,cyclin D1,Epigenetics & Nuclear Signaling,Cancer,Signal Transduction,PI3K-Akt Signaling Pathway,Cell Biology & Developmental Biology,Cell Cycle,Cyclins,G1/S Checkpoint,Hedgehog Signaling Pathway,CCND1

Molecular Weight: 33 kDa

Gene ID: 595

UniProt: P24385

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling