SMAD1 antibody (AA 20-240)

Catalog No. ABIN3021439

Product Details anti-SMAD1 Antibody

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Binding Specificity: AA 20-240

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Sequence: WKQGDEEEKW AEKAVDALVK KLKKKKGAME ELEKALSCPG QPSNCVTIPR SLDGRLQVSH RKGLPHVIYC RVWRWPDLQS HHELKPLECC EFPFGSKQKE VCINPYHYKR VESPVLPPVL VPRHSEYNPQ HSLLAQFRNL GQNEPHMPLN ATFPDSFQQP NSHPFPHSPN SSYPNSPGSS SSTYPHSPTS SDPGSPFQMP ADTPPPAYLP PEDPMTQDGS Q

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-240 of human Smad1 (NP_001003688.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-SMAD1 antibody (ABIN3021439)

Kong, Liang, Chen, Yang, Liu, Li, Feng, Wu, Liu, Tang, Yu, Ou, Lu, Yan: "Hyaluronan negatively regulates vascular calcification involving BMP2 signaling." in: Laboratory investigation; a journal of technical methods and pathology, Vol. 98, Issue 10, pp. 1320-1332, (2018) (PubMed).

Target Details for SMAD1

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Alternative Name: SMAD1 (SMAD1 Products)

Background: The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed.,BSP-1,BSP1,JV4-1,JV41,MADH1,MADR1,Smad1,SMAD1,Epigenetics & Nuclear Signaling,Transcription Factors,Cancer,Signal Transduction,Cell Biology & Developmental Biology,Growth factor,TGF-b-Smad Signaling Pathway,ESC Pluripotency and Differentiation,Immunology & Inflammation,Stem Cells,SMADs,SMAD1

Molecular Weight: 15 kDa/52 kDa

Gene ID: 4086

UniProt: Q15797

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development