FZD2 antibody (Internal Region)

Catalog No. ABIN302136

Product Details anti-FZD2 Antibody

Target: FZD2 (Frizzled Family Receptor 2 (FZD2))

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This FZD2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Purpose: Frizzled 2

Sequence: RYATLEHPFH CPR

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Grade: Verified

Immunogen: Peptide with sequence RYATLEHPFHCPR, from the internal region of the protein sequence according to NP_001457.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Thyroid Gland shows cytoplasm and membranous staining in epithelial cells of the follicles. Recommended concentration: 2-5 μg/mL. Paraffin embedded Human Liver. Recommended concentration: 2.5 μg/mL.

Western Blot: Approx 60-65 kDa band observed in Human Heart and Placenta lysates (calculated MW of 63.6 kDa according to NP_001457.1) An additional band of approx. 24 kDa was also observed in some lysates which was successfully blocked by incubation with

Peptide ELISA: antibody detection limit dilution 1:64000.

Comment:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of U2OS cells. Recommended concentration: 10μg/ml.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

References for anti-FZD2 antibody (ABIN302136)

Hirano, Yonezawa, Yunoue, Habu, Uchida, Yoshioka, Kishida, Kishida, Oyoshi, Fujio, Sugata, Yamahata, Hanaya, Arita: "Immunoreactivity of Wnt5a, Fzd2, Fzd6, and Ryk in glioblastoma: evaluative methodology for DAB chromogenic immunostaining." in: Brain tumor pathology, Vol. 31, Issue 2, pp. 85-93, (2014) (PubMed).

Target Details for FZD2

Target: FZD2 (Frizzled Family Receptor 2 (FZD2))

Alternative Name: FZD2 (FZD2 Products)

Synonyms: BG01711 antibody, CG9739 antibody, D-Fz2 antibody, D-fz2 antibody, DFZ2 antibody, DFz-2 antibody, DFz2 antibody, Dfrz2 antibody, Dfz2 antibody, Dfz[[2]] antibody, Dm Fz2 antibody, Dmel\\CG9739 antibody, FZ2 antibody, Fz2 antibody, dFz2 antibody, dfz(2) antibody, dfz2 antibody, dfz2r antibody, frz2 antibody, fz-2 antibody, fz8 antibody, zfz2 antibody, zg08 antibody, cb383 antibody, wu:fb70g09 antibody, FZD2 antibody, fzE2 antibody, hFz2 antibody, Fz-10 antibody, Fzd10 antibody, cFz-2 antibody, frizzled2 antibody, fz2 antibody, xfz2 antibody, AL033370 antibody, AW456835 antibody, Fz10 antibody, Mfz10 antibody, Mfz10a antibody, frizzled 2 antibody, frizzled class receptor 2 antibody, frizzled class receptor 2 S homeolog antibody, fz2 antibody, fzd2 antibody, FZD2 antibody, Fzd2 antibody, fzd2.S antibody

Background: FZD2 , frizzled homolog 2 (Drosophila) , frizzled 2

Gene ID: 2535, 57265, 64512

NCBI Accession: NP_001457

Pathways: WNT Signaling