CD79a antibody (AA 202-216)

Catalog No. ABIN302099

Product Details anti-CD79a Antibody

Target: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Binding Specificity: AA 202-216

Reactivity: Human, Mouse, Rat, Cow, Pig, Chicken, Guinea Pig, Horse, Rabbit, Opossum

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD79a antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: The antibody HM57 interacts with intracellular domain of CD79a (Ig alpha), a 40-45 kDa subunit of B cell antigen-specific receptor (BCR) and its early developmental forms.

Cross-Reactivity (Details): Human, Porcine, Mouse, Rat, Bovine, Equine (Horse), Guinea pig, Opossum, Rabbit, Chicken, Other not determined

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Synthetic peptide corresponding to amino acids 202-216 of human CD79a

Clone: HM57

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 5 μg/mL. Intracellular staining.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/mL.
Immunohistochemistry (frozen sections): Recommended dilution: 10 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD79a antibody (ABIN302099)

Faldyna, Samankova, Leva, Cerny, Oujezdska, Rehakova, Sinkora: "Cross-reactive anti-human monoclonal antibodies as a tool for B-cell identification in dogs and pigs." in: Veterinary immunology and immunopathology, Vol. 119, Issue 1-2, pp. 56-62, (2007) (PubMed).

Mason, Cordell, Brown, Borst, Jones, Pulford, Jaffe, Ralfkiaer, Dallenbach, Stein: "CD79a: a novel marker for B-cell neoplasms in routinely processed tissue samples." in: Blood, Vol. 86, Issue 4, pp. 1453-9, (1995) (PubMed).

Jones, Cordell, Beyers, Tse, Mason: "Detection of T and B cells in many animal species using cross-reactive anti-peptide antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 150, Issue 12, pp. 5429-35, (1993) (PubMed).

Mason, van Noesel, Cordell, Comans-Bitter, Micklem, Tse, van Lier, van Dongen: "The B29 and mb-1 polypeptides are differentially expressed during human B cell differentiation." in: European journal of immunology, Vol. 22, Issue 10, pp. 2753-6, (1992) (PubMed).

Mason, Cordell, Tse, van Dongen, van Noesel, Micklem, Pulford, Valensi, Comans-Bitter, Borst: "The IgM-associated protein mb-1 as a marker of normal and neoplastic B cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 147, Issue 11, pp. 2474-82, (1991) (PubMed).

Target Details for CD79a

Target: CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))

Alternative Name: CD79a (CD79A Products)

Synonyms: CD79A antibody, Ig-alpha antibody, Iga antibody, Igalpha antibody, Ly-54 antibody, Ly54 antibody, mb-1 antibody, IGA antibody, MB-1 antibody, IG-alpha antibody, CD79a molecule antibody, CD79A antigen (immunoglobulin-associated alpha) antibody, Cd79a molecule-like antibody, CD79A antibody, Cd79a antibody, Cd79al antibody

Background: CD79a molecule,CD79a (Ig alpha, MB1) forms disulfide-linked heterodimer with CD79b (Ig beta). They both are transmembrane proteins with extended cytoplasmic domains containing immunoreceptor tyrosine activation motives (ITAMs), and together with cell surface immunoglobulin they constitute B-cell antigen-specific receptor (BCR). CD79a and b are the first components of BCR that are expressed developmentally. They appear on pro-B cells in association with the endoplasmic reticulum chaperone calnexin. Subsequently, in pre-B cells, CD79 heterodimer is associated with lambda5-VpreB surrogate immunoglobulin and later with antigen-specific surface immunoglobulins. At the plasma cell stage, CD79a is present as an intracellular component. CD79a/b complex interacts with Src-family tyrosine kinase Lyn, which phosphorylates its cytoplasmic ITAM motives to form docking sites for downstream signaling.,BCR alpha, Ig-alpha, MB-1, IGA

Gene ID: 973

UniProt: P11912

Pathways: BCR Signaling