Keratin 10/13 antibody

Catalog No. ABIN302093

Product Details

Target: Keratin 10/13

Reactivity: Human, Cat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: The mouse monoclonal antibody DE-K13 reacts with cytokeratin 10 (56.5 kDa) and cytokeratin 13 (53 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins, represented in epithelial tissues. DE-K13 recognizes only cytokeratin 13 on formalin-fixed, paraffin-embedded tissue sections.

Cross-Reactivity (Details): Human, Feline (Cat), Other species Not tested

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Cytoskeletal preparation extracted from human ectocervical epithelium.

Clone: DE-K13

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry (paraffin sections): Standard ABC technique (DAB+), recommended dilution: 10 μg/mL (1:100), positive tissue: tonsil. Pretreatment: 0.1 % pepsin in 0.1 M HCl for 30 min at room temperature or Target Retrieval Solution - High pH . The antibody DE-K13 reacts exclusively with Cytokeratin 13.
Immunohistochemistry (frozen sections): Use at an assay dependent dilution.
Western blotting: recommended dilution: 1-3 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References

Kozaki, Nakamura, Iguchi, Kano, Watanabe, Fujiwara, Hasegawa: "Immunohistochemical analysis of cytokeratin expression in dog skin." in: The Journal of veterinary medical science / the Japanese Society of Veterinary Science, Vol. 63, Issue 1, pp. 1-4, (2001) (PubMed).

Ivanyi, Minke, Hageman, Groeneveld, van Doornewaard, Misdorp: "Cytokeratins as markers of initial stages of squamous metaplasia in feline mammary carcinomas." in: American journal of veterinary research, Vol. 54, Issue 7, pp. 1095-102, (1993) (PubMed).

Ivanyi, Minke, Hageman, Groeneveld, van Doornewaard: "Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells." in: American journal of veterinary research, Vol. 53, Issue 3, pp. 304-14, (1992) (PubMed).

Target Details

Target: Keratin 10/13

Alternative Name: Cytokeratin 10+13

Background: Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratins 10 and 13 both belong to type I family (acidic cytokeratins).,K10, K13, CK10, CK13, CK-10, CD13, CYK10, CYK13, KRT10, KRT13