CD24 antibody

Catalog No. ABIN302076

Product Details anti-CD24 Antibody

Target: CD24 (CD24 Molecule (CD24))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD24 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Cytometry by Time of Flight (CyTOF)

Specificity: The antibody SN3 reacts with CD24, a 35-45 kDa heavily glycosylated cell surface antigen. CD24 is expressed by granulocytes, B lymphocytes and by some activated T cells and T cell malignancies. It is not expressed on human thymocytes.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Glycoproteins purified from human NALM-1 cell line.

Clone: SN3

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry (frozen sections): Positive tissue: tonsil.
Flow cytometry: Recommended dilution: 1-12 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD24 antibody (ABIN302076)

Všianská, Říhová, Varmužová, Suská, Kryukov, Mikulášová, Kupská, Penka, Pour, Adam, Hájek: "Analysis of B-cell subpopulations in monoclonal gammopathies." in: Clinical lymphoma, myeloma & leukemia, Vol. 15, Issue 4, pp. e61-71, (2015) (PubMed).

Maliar, Servais, Waks, Chmielewski, Lavy, Altevogt, Abken, Eshhar: "Redirected T cells that target pancreatic adenocarcinoma antigens eliminate tumors and metastases in mice." in: Gastroenterology, Vol. 143, Issue 5, pp. 1375-84.e1-5, (2012) (PubMed).

Solvason, Kearney: "The human fetal omentum: a site of B cell generation." in: The Journal of experimental medicine, Vol. 175, Issue 2, pp. 397-404, (1992) (PubMed).

Fischer, Majdic, Gadd, Knapp: "Signal transduction in lymphocytic and myeloid cells via CD24, a new member of phosphoinositol-anchored membrane molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 2, pp. 638-41, (1990) (PubMed).

Barcos, Pollard, Fukukawa, Seon: "Follicular mantle zone cell subpopulations detected by monoclonal antibody SN3." in: Hematological oncology, Vol. 4, Issue 4, pp. 251-9, (1987) (PubMed).

Fukukawa, Matsuzaki, Haruta, Hara, Seon: "New monoclonal antibodies SN3, SN3a, and SN3b directed to sialic acid of glycoprotein on human non-T leukemia cells." in: Experimental hematology, Vol. 14, Issue 9, pp. 850-5, (1986) (PubMed).

Target Details for CD24

Target: CD24 (CD24 Molecule (CD24))

Alternative Name: CD24 (CD24 Products)

Synonyms: CD24A antibody, Cd24a antibody, CD24 molecule antibody, CD24 antibody, Cd24 antibody

Background: CD24 Molecule,CD24, also known as heat-stable antigen (HSA) or nectadorin, is a small mucin-like GPI-anchored extracellular membrane glycoprotein expressed on several cell types, including B cells. When B cells are activated and induced to further maturation, however, CD24 begins to disappear. CD24 seems to act as a gate-keeper for lipid rafts, thereby regulating the activity of integrins and other proteins such as the chemokine receptor CXCR4, it is also a ligand for P-selectin. CD24 triggering induces apoptosis of B cell precursors but not in mature resting B cells, where it instead inhibits their ability to proliferate in response to activation. CD24 expression is associated with invasiveness and poorer prognosis of carcinomas and is a marker of exosomes secreted into urine and amniotic fluid.,HSA, nectadorin, SCLC4

Gene ID: 100133941

UniProt: P25063

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation