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LAT antibody (Cytoplasmic Domain)

Name: LAT antibody (Cytoplasmic Domain)
Brand: antibodies-online
SKU: ABIN302069
Price: 366.6 USD
Availability: InStock

LAT Reactivity: Human WB, IP, FACS Host: Mouse Monoclonal LAT-01 unconjugated

Western blotting analysis of human LAT using mouse monoclonal antibody LAT-01 in lysates of Jurkat cells (positive) and Raji cells (negative control) under non-reducing and reducing conditions. (LAT antibody (Cytoplasmic Domain))

Flow cytometry intracellular staining pattern of human peripheral whole blood using anti-LAT (LAT-01) purified antibody (concentration in sample 1 μg/mL, GAM APC). (LAT antibody (Cytoplasmic Domain))

FACS

Flow Cytometry analysis of LAT expression in peripheral blood lymphocytes using anti-human LAT (LAT-01) (surface markers were stained prior to intracellular staining of human LAT). (LAT antibody (Cytoplasmic Domain))

FACS

Separation of human CD3 positive LAT positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood stained using anti-LAT (LAT-01) purified antibody (concentration in sample 1 μg/mL, GAM APC). (LAT antibody (Cytoplasmic Domain))

FACS

Flow cytometry multicolor intracellular staining of human peripheral whole blood stained using anti-LAT (LAT-01) purified antibody (concentration in sample 1 μg/mL, GAM APC) and anti-human CD3 (UCHT1) Pacific Blue antibody (20 μL reagent / 100 μL of peripheral whole blood). (LAT antibody (Cytoplasmic Domain))

FACS

5 images

(2 references)

Catalog No. ABIN302069

Datasheet as PDF

Target See all LAT Antibodies

LAT (Linker For Activation of T Cells (LAT))
Binding Specificity
21

19

15

8

8

7

7

4

4

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Cytoplasmic Domain
Reactivity
133

60

43

1
Human
Host
126

8

2

1
Mouse
Compatible Secondaries
Clonality
129

8
Monoclonal
Conjugate
67

6

4

4

4

4

4

4

4

3

3

3

3

3

3

3

3

3

3

3

3
This LAT antibody is un-conjugated
Application
75

46

39

39

16

13

9

9

4

2

1

1
Western Blotting (WB), Immunoprecipitation (IP), Flow Cytometry (FACS)

Specificity

The antibody LAT-01 reacts with an intracellular epitope of LAT, a 36-38 kDa transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets.

No Cross-Reactivity

Mouse

Cross-Reactivity (Details)

Human

Purification

Purified by protein-A affinity chromatography.

Purity

> 95 % (by SDS-PAGE)

Immunogen

Bacterially produced recombinant polypeptide corresponding to the entire cytoplasmic domain of human LAT.

Clone

LAT-01

Isotype

IgG1
anti-Linker For Activation of T Cells (LAT) antibody
LAT Reactivity: Human, Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Linker For Activation of T Cells (LAT) (pTyr191) antibody
LAT Reactivity: Human WB, ELISA, IHC, IP, IF Host: Rabbit Monoclonal 2E4 unconjugated Recombinant Antibody

anti-Linker For Activation of T Cells (LAT) (AA 1-233) antibody
LAT Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 3B11-1D5 unconjugated

anti-Linker For Activation of T Cells (LAT) (AA 30-237) antibody
LAT Reactivity: Mouse WB, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-Linker For Activation of T Cells (LAT) (pTyr161) antibody
LAT Reactivity: Human ELISA, IF (cc), IF (p), IHC (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Linker For Activation of T Cells (LAT) (Cytoplasmic Domain) antibody
LAT Reactivity: Human WB, IHC, IP, IHC (p) Host: Mouse Monoclonal LAT1111 unconjugated

anti-Linker For Activation of T Cells (LAT) (AA 25-221) antibody
LAT Reactivity: Human WB, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-Linker For Activation of T Cells (LAT) (AA 33-227) antibody
LAT Reactivity: Rat WB, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-Linker For Activation of T Cells (LAT) antibody
LAT Reactivity: Human, Mouse, Rat WB, ELISA Host: Rabbit Polyclonal unconjugated

anti-Linker For Activation of T Cells (LAT) (Tyr255) antibody
LAT Reactivity: Human WB, ELISA Host: Rabbit Polyclonal unconjugated

Application Notes

Immunoprecipitation: Tyrosine-phosphorylated form of LAT is not optimally recognized.
Flow cytometry: Recommended dilution: 1-4 μg/mL, intracellular staining. Tyrosine-phosphorylated form of LAT is not optimally recognized.
Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions

For Research Use only
Concentration

1 mg/mL

Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Do not freeze.

Storage

4 °C

Storage Comment

Store at 2-8°C. Do not freeze.
Hrdinka, Dráber, Stepánek, Ormsby, Otáhal, Angelisová, Brdicka, Paces, Horejsí, Drbal: "PRR7 is a transmembrane adaptor protein expressed in activated T cells involved in regulation of T cell receptor signaling and apoptosis." in: The Journal of biological chemistry, Vol. 286, Issue 22, pp. 19617-29, (2011) (PubMed).

Tedoldi, Paterson, Hansmann, Natkunam, Rüdiger, Angelisova, Du, Roberton, Roncador, Sanchez, Pozzobon, Masir, Barry, Pileri, Mason, Marafioti, Horejsí: "Transmembrane adaptor molecules: a new category of lymphoid-cell markers." in: Blood, Vol. 107, Issue 1, pp. 213-21, (2005) (PubMed).
Target

LAT (Linker For Activation of T Cells (LAT))

Alternative Name

LAT (LAT Products)

Synonyms

LAT antibody, lat antibody, LAT1 antibody, pp36 antibody, linker for activation of T-cells antibody, linker for activation of T cells antibody, LAT antibody, lat antibody, Lat antibody

Background

Linker for activation of T cells,LAT (linker for activation of T cells) is a 36-38 kDa double-palmitoylated transmembrane adaptor protein expressed by T cells, pre-B cells, NK cells, mast cells and platelets. After immunoreceptor triggering, LAT becomes multiply tyrosine-phosphorylated by Syk-, Src-, or Tec-family kinases, providing docking sites for downstream signaling molecules. LAT is essential for TCR-dependent T cell- and FcepsilonRI-dependent mast cell activation, as well as for maturation of early thymocytes. It is also involved in NK cell signaling and platelet activation.,IMD52

Gene ID

27040

UniProt

O43561

Pathways

TCR Signaling, Fc-epsilon Receptor Signaling Pathway