HLA-DR1 antibody (PE)
HLA-DR1
Reactivity: Human
FACS
Host: Mouse
Monoclonal
MEM-267
PE
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- Target See all HLA-DR1 products
- HLA-DR1 (MHC Class II HLA-DR1 (HLA-DR1))
- Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This HLA-DR1 antibody is conjugated to PE
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Application
- Flow Cytometry (FACS)
- Specificity
- The antibody MEM-267 specifically binds extracellularly to the empty but not peptide-loaded form of HLA-DR1. DR is the isotypes of human MHC Class II molecules expressed on antigen-presenting cells (APC, dendritic cells, B lymphocytes, monocytes, macrophages).
- Cross-Reactivity (Details)
- Human
- Purification
- Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
- Immunogen
- Purified, insoluble DR1 beta chain (DRB1*0101) expressed in E. coli inclusion bodies.
- Clone
- MEM-267
- Isotype
- IgG2b
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anti-MHC Class II HLA-DR1 (HLA-DR1) antibody
HLA-DR1 Reactivity: Human FACS, ELISA, WB Host: Mouse Monoclonal MEM-267 unconjugated
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- Application Notes
- Flow cytometry: The antibody MEM-267 stains immature dendritic cells that express empty cell surface MHC molecules, but not cells that express predominantly peptide loaded forms. Recommended dilution: 1-5 μg/mL.
- Comment
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The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
- Restrictions
- For Research Use only
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- Concentration
- 0.1 mg/mL
- Buffer
- Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Do not freeze.
Avoid prolonged exposure to light. - Storage
- 4 °C
- Storage Comment
- Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
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- Target
- HLA-DR1 (MHC Class II HLA-DR1 (HLA-DR1))
- Alternative Name
- HLA-DR1 (HLA-DR1 Products)
- Background
- HLA-DR1 belongs to the HLA class II beta chain paralogues. The MHC Class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. MHC Class II molecules are expressed in antigen presenting cells (APC). The beta chain is approximately 26-28 kDa. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation.
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