CD86 antibody (APC)

Catalog No. ABIN301986

Product Details anti-CD86 Antibody (APC)

Target: CD86

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD86 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Specificity: The mouse monoclonal antibody BU63 reacts with an extracellular epitope of CD86 (B7-2), a 70 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.

Cross-Reactivity (Details): Human, Other not determined

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: B-lymphoblastoid cell line ARH 77

Clone: BU63

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

Comment:

The purified antibody is conjugated with cross-linked Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use. No reconstitution is necessary.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-CD86 antibody (APC) (ABIN301986)

Hovden, Karlsen, Jonsson, Aarstad, Appel: "Maturation of monocyte derived dendritic cells with OK432 boosts IL-12p70 secretion and conveys strong T-cell responses." in: BMC immunology, Vol. 12, pp. 2, (2011) (PubMed).

Kolar, Mehta, Pelayo, Capra: "A novel human B cell subpopulation representing the initial germinal center population to express AID." in: Blood, Vol. 109, Issue 6, pp. 2545-52, (2007) (PubMed).

Chan, Baird, Mercer, Fleming: "Maturation and function of human dendritic cells are inhibited by orf virus-encoded interleukin-10." in: The Journal of general virology, Vol. 87, Issue Pt 11, pp. 3177-81, (2006) (PubMed).

Zhan, Towler, Calder: "The immunomodulatory role of human conjunctival epithelial cells." in: Investigative ophthalmology & visual science, Vol. 44, Issue 9, pp. 3906-10, (2003) (PubMed).

Mauri, Wyss-Coray, Gallati, Pichler: "Antigen-presenting T cells induce the development of cytotoxic CD4+ T cells. I. Involvement of the CD80-CD28 adhesion molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 155, Issue 1, pp. 118-27, (1995) (PubMed).

Caux, Vanbervliet, Massacrier, Azuma, Okumura, Lanier, Banchereau: "B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells." in: The Journal of experimental medicine, Vol. 180, Issue 5, pp. 1841-7, (1994) (PubMed).

Engel, Gribben, Freeman, Zhou, Nozawa, Abe, Nadler, Wakasa, Tedder: "The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen." in: Blood, Vol. 84, Issue 5, pp. 1402-7, (1994) (PubMed).

Target Details for CD86

Target: CD86

Alternative Name: CD86 (CD86 Products)

Synonyms: B7-2 antibody, B7.2 antibody, B70 antibody, CD28LG2 antibody, LAB72 antibody, B7 antibody, CLS1 antibody, Cd28l2 antibody, ETC-1 antibody, Ly-58 antibody, Ly58 antibody, MB7 antibody, MB7-2 antibody, TS/A-2 antibody, CD86 molecule antibody, CD86 antigen antibody, integrin subunit alpha 2b antibody, CD86 antibody, Cd86 antibody, Itga2b antibody

Background: CD86 Molecule,CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 Molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet, there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.,B7-2, FUN-1, LAB72

Gene ID: 942

UniProt: P42081

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation