RUNX2 antibody (AA 242-521)

Catalog No. ABIN3016834

Product Details anti-RUNX2 Antibody

Target: RUNX2 (Runt-Related Transcription Factor 2 (RUNX2))

Binding Specificity: AA 242-521

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Sequence: FSDRLSDLGR IPHPSMRVGV PPQNPRPSLN SAPSPFNPQG QSQITDPRQA QSSPPWSYDQ SYPSYLSQMT SPSIHSTTPL SSTRGTGLPA ITDVPRRISD DDTATSDFCL WPSTLSKKSQ AGASELGPFS DPRQFPSISS LTESRFSNPR MHYPATFTYT PPVTSGMSLG MSATTHYHTY LPPPYPGSSQ SQSGPFQTSS TPYLYYGTSS GSYQFPMVPG GDRSPSRMLP PCTTTSNGST LLNPNLPNQN DGVDADGSHS SSPTVLNSSG RMDESVWRPY

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 242-521 of human RUNX2 (NP_001019801.3).

Isotype: IgG

Application Details

Application Notes: WB,1:200 - 1:1000,IHC,1:50 - 1:200,IF,1:50 - 1:200

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-RUNX2 antibody (ABIN3016834)

Zhang, Wang, Zeng, Yan, Ouyang, Liu, Sun, Zhao, Fang, Pan, Xie, Yang, Zhang, Bai, Cai: "mTORC1 activation downregulates FGFR3 and PTH/PTHrP receptor in articular chondrocytes to initiate osteoarthritis." in: Osteoarthritis and cartilage, Vol. 25, Issue 6, pp. 952-963, (2018) (PubMed).

Gao, Li, Li: "Exendin-4 promotes the osteogenic differentiation of osteoblasts via the Hedgehog/Gli1 signaling pathway." in: American journal of translational research, Vol. 10, Issue 1, pp. 315-324, (2018) (PubMed).

Wang, Zhang, Sun, Wang, Yang, Yang, Zhang, Luo, Wang, Jiang, Zeng, Cai, Bai: "Intra-articular Delivery of Antago-miR-483-5p Inhibits Osteoarthritis by Modulating Matrilin 3 and Tissue Inhibitor of Metalloproteinase 2." in: Molecular therapy : the journal of the American Society of Gene Therapy, Vol. 25, Issue 3, pp. 715-727, (2017) (PubMed).

Li, Xu, Xing, Qian, Chen, Gu, Guo, Lai, Zhao, Li, Wang, Wang, Deng: "A Conditional Knockout Mouse Model Reveals a Critical Role of PKD1 in Osteoblast Differentiation and Bone Development." in: Scientific reports, Vol. 7, pp. 40505, (2017) (PubMed).

Wu, Wang, Ji, Ou, Xia, Zhang, Zhao: "C4orf7 modulates osteogenesis and adipogenesis of human periodontal ligament cells." in: American journal of translational research, Vol. 9, Issue 12, pp. 5708-5718, (2017) (PubMed).

Target Details for RUNX2

Target: RUNX2 (Runt-Related Transcription Factor 2 (RUNX2))

Alternative Name: RUNX2 (RUNX2 Products)

Synonyms: AML3 antibody, CBF-alpha-1 antibody, CBFA1 antibody, CCD antibody, CCD1 antibody, CLCD antibody, OSF-2 antibody, OSF2 antibody, PEA2aA antibody, PEBP2aA antibody, Cbf antibody, Cbfa-1 antibody, Cbfa1 antibody, LS3 antibody, Osf2 antibody, Pebp2a1 antibody, Pebpa2a antibody, runx2 antibody, RUNX2 antibody, ccd antibody, aml3 antibody, ccd1 antibody, osf2 antibody, cbfa1 antibody, pea2aa antibody, pebp2a1 antibody, pebp2a2 antibody, pebp2aa antibody, pebp2aa1 antibody, runt related transcription factor 2 antibody, runt-related transcription factor 2a antibody, runt-related transcription factor 2 antibody, runt related transcription factor 2 L homeolog antibody, RUNX2 antibody, runx2a antibody, Runx2 antibody, runx2 antibody, LOC703331 antibody, LOC100549663 antibody, runx2.L antibody

Background: This gene is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis and acts as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Two regions of potential trinucleotide repeat expansions are present in the N-terminal region of the encoded protein, and these and other mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants that encode different protein isoforms result from the use of alternate promoters as well as alternate splicing.,RUNX2,AML3,CBF-alpha-1,CBFA1,CCD,CCD1,CLCD,OSF-2,OSF2,PEA2aA,PEBP2aA,Epigenetics & Nuclear Signaling,Transcription Factors,Cell Biology & Developmental Biology,Extracellular Matrix,Bone,Hippo Signaling Pathway,Stem Cells,Mesenchymal Stem Cells,Target gene,RUNX2

Molecular Weight: 54 kDa/55 kDa/56 kDa

Gene ID: 860

UniProt: Q13950