GPX4 antibody (AA 30-197)

Catalog No. ABIN3015730

Product Details anti-GPX4 Antibody

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Binding Specificity: AA 30-197

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPX4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Sequence: ASRDDWRCAR SMHEFSAKDI DGHMVNLDKY RGFVCIVTNV ASQUGKTEVN YTQLVDLHAR YAECGLRILA FPCNQFGKQE PGSNEEIKEF AAGYNVKFDM FSKICVNGDD AHPLWKWMKI QPKGKGILGN AIKWNFTKFL IDKNGCVVKR YGPMEEPLVI EKDLPHYF

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-197 of human GPX4 (NP_002076.2).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:50 - 1:200,IP,1:50 - 1:200

Restrictions: For Research Use only

Handling

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

References for anti-GPX4 antibody (ABIN3015730)

Wang, Zheng, Zhang, Zhao, Wang, Wang, Shang, Barasch, Qiu: "Physiological functions of ferroportin in the regulation of renal iron recycling and ischemic acute kidney injury." in: American journal of physiology. Renal physiology, Vol. 315, Issue 4, pp. F1042-F1057, (2018) (PubMed).

Target Details for GPX4

Target: GPX4 (Glutathione Peroxidase 4 (GPX4))

Alternative Name: GPX4 (GPX4 Products)

Background: The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of hydrogen peroxide, organic hydroperoxides and lipid hydroperoxides, and thereby protect cells against oxidative damage. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme has a high preference for lipid hydroperoxides and protects cells against membrane lipid peroxidation and cell death. It is also required for normal sperm development, thus, it has been identified as a 'moonlighting' protein because of its ability to serve dual functions as a peroxidase, as well as a structural protein in mature spermatozoa. Mutations in this gene are associated with Sedaghatian type of spondylometaphyseal dysplasia (SMDS). This isozyme is also a selenoprotein, containing the rare amino acid selenocysteine (Sec) at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been found for this gene.,GPX4,GPx-4,GSHPx-4,MCSP,PHGPx,SMDS,snGPx,snPHGPx,Cancer,Signal Transduction,Endocrine & Metabolism,Mitochondrial metabolism,Mitochondrial markers,GPX4

Molecular Weight: 19 kDa/22 kDa

Gene ID: 2879

UniProt: P36969