SIK2 antibody (AA 100-150)

Catalog No. ABIN297633

Product Details anti-SIK2 Antibody

Target: SIK2 (Salt-Inducible Kinase 2 (SIK2))

Binding Specificity: AA 100-150

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SIK2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human SIK2 is a 926 amino acid protein. The approximately 120 kD band is higher than the theoretical expected size, but this band, as well as the approximately 25 kD lower band, are blocked by immunizing peptide.

Purification: Protein G purified

Immunogen: A portion of amino acids 100-150 of human SIK2.

Type of Immunogen: Protein fragment

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (1 - 3 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.05 % BSA, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: avoid freeze thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.

Target Details for SIK2

Target: SIK2 (Salt-Inducible Kinase 2 (SIK2))

Alternative Name: SIK2 / SNF1LK2 (SIK2 Products)

Background: Name/Gene ID: SIK2
Subfamily: CAMKL
Family: Protein Kinase

Synonyms: SIK2, KIAA0781, LOH11CR1I, SNF1LK2, QIK, Qin-induced kinase, Salt-inducible kinase 2, SIK-2, SNF1-like kinase 2

Gene ID: 23235

UniProt: Q9H0K1