ITGA4 antibody

Catalog No. ABIN2967175

Product Details anti-ITGA4 Antibody

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Reactivity: Human, Cow, Dog, Horse, Cat, Sheep, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This ITGA4 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Specificity: The mouse monoclonal antibody 9F10 recognizes an extracellular epitope of CD49d (alpha 4 integrin), a 145-180 kDa type I transmembrane glycoprotein expressed on B and T cells, monocytes, eosinophils, basophils, NK cells, and dendritic cells, but not platelets.

Cross-Reactivity (Details): Human, Non-Human Primates, Bovine, Canine (Dog), Equine (Horse), Feline (Cat), Sheep

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Endotoxin Level: Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.

Clone: 9F10

Isotype: IgG1 kappa

Application Details

Application Notes: Functional application: In vitro T cell costimulation.
Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4

Preservative: Azide free

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for ITGA4

Target: ITGA4 (Integrin alpha 4 (ITGA4))

Alternative Name: CD49d / Integrin alpha 4 (ITGA4 Products)

Synonyms: CD49D antibody, IA4 antibody, LOC100219853 antibody, integrin subunit alpha 4 antibody, integrin alpha 4 antibody, integrin subunit alpha 4 S homeolog antibody, ITGA4 antibody, Itga4 antibody, itga4.S antibody

Background: Integrin subunit alpha 4,CD49d / integrin alpha 4, unlike other alpha integrins, neither contains an I-domain, nor undergoes disulfide-linked cleavage. It associates with beta 7 chain to form alpha 4 / beta 7 integrin, and with beta 1 chain (CD29) to form VLA-4 integrin. These complexes are important for lymphocyte migration from circulation into tissue (binding VCAM-1) and homing of T cell subsets to Peyer´s patches (binding MadCAM-1), but VLA-4 is also target for invasive bacteria which contain invasin. CD49d is essential for differentiation and migration of hematopoietic stem cells by their adhesion to bone marrow stromal cells, and provides a costimulatory signal to TCR-CD3 complex by inducing phosphorylation of some focal adhesion proteins.,ITGA4, VLA-4 alpha

Gene ID: 3676

UniProt: P13612

Pathways: Integrin Complex