Keratin Acidic (AE1) antibody

Catalog No. ABIN2967160

Product Details

Target: Keratin Acidic (AE1)

Reactivity: Human, Rat, Rabbit, Chicken, Mouse, Dog, Cat, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Mouse monoclonal antibody AE1 recognizes acidic type cytokeratins (intracellular antigens), namely K10, 14, 15, 16, 19 (40-56 kDa). This antibody stains well the basal layer of epidermis and most epithelia.

Cross-Reactivity (Details): Human, Non-Human Primates, Mouse, Rat, Canine (Dog), Feline (Cat), Rabbit, Chicken

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human epidermal keratins

Clone: AE1

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.
Western blotting: Recommended dilution: 1-2 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details

Target: Keratin Acidic (AE1)

Alternative Name: Acidic Cytokeratins (AE1 Products)

Background: Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. CThey are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,Type I cytokeratins