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Keratin Acidic (AE1) antibody

AE1 Reactivity: Human, Rat, Rabbit, Chicken, Mouse, Dog, Cat, Non-Human Primate WB, FACS, IHC (p), ICC, IHC (fro) Host: Mouse Monoclonal AE1 unconjugated
Catalog No. ABIN2967160
  • Target See all Keratin Acidic (AE1) Antibodies
    Keratin Acidic (AE1)
    Reactivity
    • 31
    • 10
    • 8
    • 8
    • 8
    • 7
    • 5
    • 5
    • 3
    • 3
    • 2
    • 1
    Human, Rat, Rabbit, Chicken, Mouse, Dog, Cat, Non-Human Primate
    Host
    • 17
    • 11
    • 3
    Mouse
    Clonality
    • 31
    Monoclonal
    Conjugate
    • 14
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    Un-conjugated
    Application
    • 24
    • 23
    • 23
    • 20
    • 11
    • 2
    • 2
    • 2
    Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    Mouse monoclonal antibody AE1 recognizes acidic type cytokeratins (intracellular antigens), namely K10, 14, 15, 16, 19 (40-56 kDa). This antibody stains well the basal layer of epidermis and most epithelia.
    Cross-Reactivity (Details)
    Human, Non-Human Primates, Mouse, Rat, Canine (Dog), Feline (Cat), Rabbit, Chicken
    Purification
    Purified by protein-A affinity chromatography.
    Purity
    > 95 % (by SDS-PAGE)
    Immunogen
    Human epidermal keratins
    Clone
    AE1
    Isotype
    IgG1
    Top Product
    Discover our top product AE1 Primary Antibody
  • Application Notes
    Flow cytometry: Recommended dilution: 1-5 μg/mL. Intracellular staining.
    Western blotting: Recommended dilution: 1-2 μg/mL.
    Restrictions
    For Research Use only
  • Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store at 2-8°C. Do not freeze.
  • Target
    Keratin Acidic (AE1)
    Alternative Name
    Acidic Cytokeratins (AE1 Products)
    Background
    Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. CThey are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,Type I cytokeratins
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