FHL1 antibody

Catalog No. ABIN7306298

Product Details anti-FHL1 Antibody

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FHL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Specificity: Recognizes endogenous levels of FHL-1 protein.

Characteristics: Rabbit polyclonal antibody to FHL-1

Purification: The antibody was purified by immunogen affinity chromatography.

Immunogen: Recombinant full length protein of human FHL-1

Application Details

Application Notes: WB (1:500 - 1:2000), IF/IC (1:50 - 1:100)

Restrictions: For Research Use only

Independent Validation (IF)

Validation #101371 (Immunofluorescence)

by: Alexander Lab, Jacobs School of Medicine and Biomedical Sciences, Department of Medicine, University of Buffalo

No.: #101371

Date: 07/19/2017

Antigen: FHL-1

Lot Number: AL1F04A

Method validated: Immunofluorescence

Positive Control: MRL/lpr lupus mice (14 weeks of age)

Negative Control: FH knockout MRL/lpr lupus mice

Notes: Passed. ABIN2966633 works well on murine kidney samples and shows the expected CFH1 localization.

Validation Images

IF staining with ABIN2966633 of kidneys harvested from 14 week old MRL/lpr mice sufficient and deficient in factor H. Cryosections were fixed, stained and images acquired as described in the protocol.

Full Methods

Primary Antibody: ABIN2966633

Secondary Antibody: goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251)

Full Protocol:

• Prepare kidneys of wt and complement factor H ko MRL/lpr lupus mice of 14 weeks of age.
• Snap-freeze kidneys in 2-methylbutane cooled on dry ice and keep at −80°C until use.
• Sections were cut at 8µm using the cryostat.
• Dry sections for 30min at RT.
• Hydrate sections in PBS 2x for 15min at RT.
• Fix cryostat sections with 4% paraformaldehyde (PFA) for 15min at RT.
• Wash sections 2x with PBS for 15min at RT.
• Incubate sections with primary rabbit anti-FHL-1 antibody (antibodies-online, ABIN2966633, AL1F04A) diluted 1:100 in PBS ON at 4°C.
• Wash sections 2x for 15min with PBS.
• Incubate sections with secondary goat anti-rabbit rhodamine conjugated antibody (ThermoFisher Scientific, T-2769, lot 6251) diluted 1:100 in PBS for 30min at RT.
• Wash sections 2x for 15min with PBS.
• Incubate sections with ActinGreen 488 ReadyProbes Reagent (ThermoFisher Scientific, R37122, lot 1700569) diluted 1:100 in PBS for 30min at RT.
• Wash sections 2x for 15min with PBS.
• Image acquisition on a Zeiss microscope (Carl Zeiss, Oberkochen, Germany).

Handling

Format: Liquid

Buffer: Liquid in 0.42 % Potassium phosphate, 0.87 % Sodium chloride, pH 7.3, 30 % glycerol, and 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Expiry Date: 12 months

Target Details for FHL1

Target: FHL1 (Four and A Half LIM Domains 1 (FHL1))

Alternative Name: FHL-1 (FHL1 Products)

Background: SLIM1, Four and a half LIM domains protein 1, FHL-1, Skeletal muscle LIM-protein 1, SLIM, SLIM-1

Gene ID: 2273, 14199, 25177

UniProt: Q13642, P97447, Q9WUH4