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LPIN3 antibody (AA 428-529)

LPIN3 Reactivity: Mouse WB, IHC, ELISA, ICC Host: Goat Polyclonal unconjugated
Catalog No. ABIN294094
  • Target See all LPIN3 Antibodies
    LPIN3 (Lipin 3 (LPIN3))
    Binding Specificity
    • 1
    • 1
    AA 428-529
    Reactivity
    • 5
    • 2
    Mouse
    Host
    • 4
    • 2
    • 1
    Goat
    Clonality
    • 4
    • 3
    Polyclonal
    Conjugate
    • 7
    This LPIN3 antibody is un-conjugated
    Application
    • 5
    • 4
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)
    Specificity
    Selected for its ability to recognize Lipin 3 in the applications listed. In direct ELISAs and Western Blots, this antibody shows ~50 % cross-reactivity with rhLipin 3 and less than 5 % cross-reactivity with rhLipin 1 and rhLipin 2.
    Purification
    Affinity purified
    Immunogen
    E. coli-derived recombinant mouse Lipin 3 (aa428-529).
    Isotype
    IgG
    Top Product
    Discover our top product LPIN3 Primary Antibody
  • Application Notes
    Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), IHC (10 μg/mL), WB (0.1 - 0.2 μg/mL)

    Usage: Suitable for use in ELISA, Western Blot, Immunocytochemistry. Western Blot: 0.1-0.2 μg/mL with the appropriate secondary reagents to detect mouse rmLipin 3. The detection limit for rmLipin 3 is approximately 2 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: 10 μg/mL to detect Lipin 3 on CCD-18co normal human colon fibroblasts. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody overnight at 4?C followed by donkey anti-goat IgG at RT in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Immunohistochemistry: 10 μg/mL to detect Lipin 3 in cryosections of adult mouse intestine. Tissue was fixed overnight in 4 % PFA + 14 % picric acid in PBS at 4?C. Sections were blocked with 10 % donkey serum and 0.1 % Triton in 1 % BSA in PBS for 45 minutes at RT. After blocking, sections were incubated with primary antibody for 3 hours at RT followed by donkey anti-goat lgG at RT for 1 hour. Between each step, sections were washed with 0.1 % BSA in PBS. Direct ELISA: 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse rmLipin 3. The detection limit for rmLipin 3 is approximately 0.05 ng/well.
    Comment

    Target Species of Antibody: Mouse

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Sterile PBS.
    Concentration
    Lot specific
    Buffer
    Lyophilized from PBS, 5 % trehalose
    Storage
    -20 °C
    Storage Comment
    Lyophilized powder may be stored at -20°C for short-term only. Reconstituted product is stable for 1 year at -20°C.
  • Target
    LPIN3 (Lipin 3 (LPIN3))
    Alternative Name
    LPIN3 / LIPIN-3 (LPIN3 Products)
    Synonyms
    MGC82840 antibody, LPIN3 antibody, DKFZp469D075 antibody, LIPN3L antibody, SMP2 antibody, dJ450M14.2 antibody, dJ450M14.3 antibody, dJ620E11.2 antibody, 9130206L11Rik antibody, AA438110 antibody, AV236139 antibody, mKIAA4023 antibody, lipin 3 L homeolog antibody, lipin 3 antibody, lpin3.L antibody, LPIN3 antibody, LOAG_08747 antibody, lpin3 antibody, Lpin3 antibody
    Background
    Name/Gene ID: LPIN3

    Synonyms: LPIN3, DJ620E11.2, DJ450M14.2, Lipin-3, Lipin 3, Lipin 3-like, Lipin-3-like, SMP2, DJ450M14.3, LIPN3L
    Gene ID
    64900
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