LacI / Lac Repressor antibody
Reactivity: E. coli, Shigella sonnei
ELISA, WB
Host: Rabbit
Polyclonal
unconjugated
1 image
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- Target
- LacI / Lac Repressor
- Reactivity
- E. coli, Shigella sonnei
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- ELISA, Western Blotting (WB)
- Specificity
- Recombinant Lac I protein. A BLAST analysis was used to suggest cross-reactivity with Lac I from DNA-binding transcriptional repressor in Escherichia coli (strain DH10B) and transcriptional repressor of the lac operon in Shigella sonnei (strain Ss046) based on a 100 % homology with the immunizing sequence. Cross-reactivity with Lac I from other sources has not been determined
- Purification
- Immunoaffinity purified
- Immunogen
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Recombinant Lac I protein.
Type of Immunogen: Recombinant protein
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- Application Notes
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Approved: ELISA (1:90000 - 1:100000), WB (1:500 - 1:2000)
Usage: This affinity purified antibody has been tested for use in ELISA and western blotting using recombinant Lac I protein. Specific conditions for reactivity and detection of Lac I should be optimized by the end user. Expect a band approximately ~38 kD in size corresponding to Lac I by western blotting in the appropriate cell lysate or extract. - Comment
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Target Species of Antibody: E. coli
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Aliquot to Avoid freeze/thaw cycles. Do not freeze diluted antibody.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Aliquot to avoid freeze-thaw cycles. Do not freeze diluted antibody.
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- Target
- LacI / Lac Repressor
- Background
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Name/Gene ID: LacI
Synonyms: LacI
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