HES4 antibody (AA 94-221)

Catalog No. ABIN291868

Product Details anti-HES4 Antibody

Target: HES4 (Hairy and Enhancer of Split 4 (HES4))

Binding Specificity: AA 94-221

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This HES4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Specificity: Selected for its ability to recognize human HES-4 in the applications listed below. In direct ELISAs and Western Blots, this antibody shows less than 1 % cross-reactivity with rhHES-1, rhHES-2, rhHES-5, and rhHES-7.

Purification: Affinity purified

Immunogen: E. coli-derived rhHES-4 (aa94-221).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA, ICC, WB

Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect human HES-4. The detection limit for rhHES-4 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect HES-4 in HEK293 cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by fluorochrome-coupled anti-sheep IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing 0.1 % BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect human HES-4. The detection limit for rhHES-4 is approximately 0.2 ng/well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Target Details for HES4

Target: HES4 (Hairy and Enhancer of Split 4 (HES4))

Alternative Name: HES4 (HES4 Products)

Background: Name/Gene ID: HES4

Synonyms: HES4, BHLHb42, Transcription factor HES-4, BHLH factor Hes4, Hairy and enhancer of split 4, HHES4

Gene ID: 57801

UniProt: Q9HCC6