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alpha Tubulin antibody (C-Term, Subunit alpha)

TUBA1 Reactivity: Saccharomyces cerevisiae, Mammalian, Avian, E. coli WB, ELISA, IHC, IP, IHC (fro), RIA Host: Rat Monoclonal unconjugated

Catalog No. ABIN291744

Datasheet as PDF

Target See all alpha Tubulin (TUBA1) Antibodies

alpha Tubulin (TUBA1)
Binding Specificity
9

8

6

5

4

4

3

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term, Subunit alpha
Reactivity
95

71

61

24

21

16

14

14

8

7

7

6

6

5

5

4

4

4

4

4

4

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1
Saccharomyces cerevisiae, Mammalian, Avian, E. coli
Host
53

45

10

2

2

1

1

1
Rat
Compatible Secondaries
Clonality
71

41

2
Monoclonal
Conjugate
84

9

7

3

2

1

1

1

1

1

1

1

1

1

1
This alpha Tubulin antibody is un-conjugated
Application
96

45

36

35

33

32

26

15

9

7

5

2

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Specificity

Recognizes the alpha subunit of tubulin, specifically binding to so called Tyr-Tubulin, which is produced by post translational modification of tubulin. The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Gly-Gly-Tyr in Tyr-Tubulin). The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin. Species cross-reactivity: Reacts with tubulin from yeast, avian and mammalian.

Purification

Protein G purified

Immunogen

Yeast tubulin.

Type of Immunogen: Purified protein

Isotype

IgG2a

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anti-alpha Tubulin (TUBA1) antibody (FITC)
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anti-alpha Tubulin (TUBA1) antibody (Biotin)
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TUBA1 Reactivity: Human, Mouse, Rat, Chicken, Cow WB, ELISA Host: Rabbit Polyclonal unconjugated

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TUBA1 Reactivity: Human, Mouse, Rat WB, ELISA, IF, IHC (p) Host: Rabbit Polyclonal unconjugated

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TUBA1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-alpha Tubulin (TUBA1) antibody
TUBA1 Reactivity: Human WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-alpha Tubulin (TUBA1) antibody
TUBA1 Reactivity: Human, Mouse, Rat, Pig, Cow, Saccharomyces cerevisiae, Monkey, Dog, Hamster, Guinea Pig, Amphibian, Goat, Sea Urchin, Kangaroo WB, IHC, IHC (p), IP Host: Mouse Monoclonal DM1A unconjugated

Application Notes

Approved: ELISA, IHC, IHC-Fr, IP, RIA, WB

Usage: Immunohistochemistry (frozen). ELISA. Immunoprecipitation. Western Blot. Radioimmunoassay.

Comment

Target Species of Antibody: Yeast

Restrictions

For Research Use only
Format

Liquid

Concentration

Lot specific

Buffer

PBS,  pH 7.2, 0.1 % sodium azide.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Target

alpha Tubulin (TUBA1)

Alternative Name

Alpha Tubulin (TUBA1 Products)

Synonyms

Tuba1 antibody, K-ALPHA-1 antibody, Tuba-1 antibody, cb944 antibody, fb22g06 antibody, tuba1 antibody, wu:fb22g06 antibody, Calpha1 antibody, tubulin, alpha 1A antibody, tubulin alpha 1b antibody, tubulin, alpha 1b antibody, tubulin, alpha 1B antibody, Tuba1a antibody, TUBA1B antibody, tuba1b antibody, Tuba1b antibody

Pathways

Microtubule Dynamics