GFP antibody

Catalog No. ABIN289429

Product Details anti-GFP Antibody

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Goat

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity (Details): Minimum cross-reactivity to Human, Mouse, and Rat Serum Proteins.1 mg/ml

Purification: Affinity chromatography purified

Sterility: Filtered

Immunogen: GFP antibody was raised in goat using a GFP fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria as the immunogen.

Application Details

Application Notes: ELISA: 1:20,000-1:100,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Sterile filtered liquid in 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, and 0.01 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at -20 °C prior to opening. Aliquot and freeze at -20 °C or below for long term storage. This product is stable for several weeks at 4 °C as an undiluted liquid.

References for anti-GFP antibody (ABIN289429)

Buser, Drubin: "Ultrastructural imaging of endocytic sites in Saccharomyces cerevisiae by transmission electron microscopy and immunolabeling." in: Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, Vol. 19, Issue 2, pp. 381-92, (2013) (PubMed).

Buser, McDonald: "Correlative GFP-immunoelectron microscopy in yeast." in: Methods in enzymology, Vol. 470, pp. 603-18, (2010) (PubMed).

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP (GFP Products)

Synonyms: green fluorescent protein antibody, gfp antibody

Background: Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.