Collagen Type V antibody
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- Target See all Collagen Type V (COL5) Antibodies
- Collagen Type V (COL5) (Collagen, Type V (COL5))
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Reactivity
- Human, Dog, Sheep
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Collagen Type V antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Specificity
- Highly specific for type V collagen. It has been shown to have no cross-reactivity with type I, III and VI collagens by ELISA and Western Blot. There is no evidence for cross-reactivity with other connective tissue proteins (laminin, fibronectin, elastin). Shows reactivity with human, canine, bovine, sheep, porcine and kangaroo type V collagens by ELISA and Western Blot. Does not show reactivity with mouse, rat, guinea pig and chicken type V collagens by ELISA and Western Blot. Can be used for detection of collagens by ELISA. Has been used successfully for immunohistology on frozen unfixed sections of human, bovine and dog skin, of fetal bovine cornea, and of new dog tissue associated with a biomaterial implant. If fixation of tissue is required, acetone or ethanol is recommended. Binds poorly to Collagen V when tested in ELISA with Collagen V coated directly onto the microtiter well.
- Purification
- Protein A/G purified
- Immunogen
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Acid-digested pepsin soluble canine type V collagen.
Type of Immunogen: Protein - Isotype
- IgG2a kappa
- Top Product
- Discover our top product COL5 Primary Antibody
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- Application Notes
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Approved: ELISA (1:50), IHC, WB
Usage: Suitable for use in ELISA, Western Blot, Immunohistochemistry. ELISA: 1:50. - Comment
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Target Species of Antibody: Dog
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, 15 mM sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- avoid freeze thaw cycles. Store undiluted.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
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- Target
- Collagen Type V (COL5) (Collagen, Type V (COL5))
- Alternative Name
- Collagen Type V (COL5 Products)
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