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GFP antibody (HRP)

GFP Reactivity: Aequorea victoria WB, ELISA, IHC Host: Goat Polyclonal HRP

Catalog No. ABIN288233

Datasheet as PDF

Target See all GFP Antibodies

GFP (Green Fluorescent Protein (GFP))
Reactivity
173

15

11

9
Aequorea victoria
Host
64

62

33

13

9

5

2

1
Goat
Compatible Secondaries
Clonality
112

72
Polyclonal
Conjugate
81

12

11

9

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is conjugated to HRP
Application
146

74

46

32

27

25

23

21

14

13

13

12

12

5

4

4

2

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity (Details)

Minimum cross reactivity to Human, Mouse and Rat serum proteins1 mg/ml

Purification

Affinity chromatography purified

Immunogen

GFP antibody (HRP) was raised in goat using a GFP fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria as the immunogen.

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anti-Green Fluorescent Protein (GFP) (AA 246) antibody
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anti-Green Fluorescent Protein (GFP) antibody
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anti-Green Fluorescent Protein (GFP) (AA 246) antibody
GFP Reactivity: Aequorea victoria WB, ELISA, FM Host: Rabbit Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM Host: Goat Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) (AA 246) antibody (FITC)
GFP Reactivity: Aequorea victoria FACS, IM Host: Goat Polyclonal FITC

anti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria WB, IP, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,000, WB: 1:2,000 - 1:5,000
Optimal conditions should be determined by the investigator.

Restrictions

For Research Use only
Format

Lyophilized

Concentration

Lot specific

Buffer

Lyophilized from 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, 10 mg/mL BSA, and 0.01 % gentamycin sulfate. DO NOT add NaN3. Immunoglobulin and protease free.

Preservative

Without preservative

Handling Advice

Avoid repeated freeze/thaw cycles.
Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Dilute only prior to immediate use.

Storage

4 °C/-20 °C

Storage Comment

Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.
Target

GFP (Green Fluorescent Protein (GFP))

Alternative Name

GFP (GFP Products)

Synonyms

green fluorescent protein antibody, gfp antibody

Background

Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.