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GFP antibody (Biotin)

GFP Reactivity: Aequorea victoria WB, ELISA, IHC Host: Goat Polyclonal Biotin

Catalog No. ABIN288232

Datasheet as PDF

Target See all GFP Antibodies

GFP (Green Fluorescent Protein (GFP))
Reactivity
175

15

11

9
Aequorea victoria
Host
64

63

34

13

9

5

2

1
Goat
Compatible Secondaries
Clonality
113

73
Polyclonal
Conjugate
82

12

11

10

5

4

3

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This GFP antibody is conjugated to Biotin
Application
148

80

47

34

27

25

23

21

14

13

13

12

12

5

4

4

4

2

1

1

1

1

1
Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purification

Affinity chromatography purified

Immunogen

GFP antibody (biotin) was raised in goat using a GFP fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria as the immunogen.

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anti-Green Fluorescent Protein (GFP) antibody
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anti-Green Fluorescent Protein (GFP) (AA 246) antibody
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GFP Reactivity: Aequorea victoria WB, ELISA, IP, IC, ChIP-seq Host: Rat Monoclonal 1A5 unconjugated

anti-Green Fluorescent Protein (GFP) antibody
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anti-Green Fluorescent Protein (GFP) (AA 246) antibody
GFP Reactivity: Aequorea victoria WB, ELISA, FM Host: Rabbit Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria WB, IF, IHC (fro), IHC (p), IEM Host: Goat Polyclonal unconjugated

anti-Green Fluorescent Protein (GFP) (AA 246) antibody (FITC)
GFP Reactivity: Aequorea victoria FACS, IM Host: Goat Polyclonal FITC

anti-Green Fluorescent Protein (GFP) antibody
GFP Reactivity: Aequorea victoria WB, IP, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

ELISA: 1:10,000-1:50,000, IHC: 1:1,000-1:5,000, WB: 1:2,000-1:10,000
Optimal conditions should be determined by the investigator.

Restrictions

For Research Use only
Format

Lyophilized

Concentration

Lot specific

Buffer

Lyophilized from 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, 10 mg/mL BSA, and 0.01 % NaN3. Immunoglobulin and protease free.

Preservative

Sodium azide

Precaution of Use

This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice

Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage

4 °C/-20 °C

Storage Comment

Store at 4 °C for short term storage. Aliquot and store at -20 °C for long term storage.
Target

GFP (Green Fluorescent Protein (GFP))

Alternative Name

GFP (GFP Products)

Synonyms

green fluorescent protein antibody, gfp antibody

Background

Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.