FITC antibody (HRP)

Catalog No. ABIN288221

Product Details anti-FITC Antibody (HRP)

Target: FITC (Fluorescein Isothiocyanate (FITC))

Reactivity: Please inquire

Host: Goat

Clonality: Polyclonal

Conjugate: This FITC antibody is conjugated to HRP

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Purification: Affinity chromatography purified

Immunogen: FITC antibody (HRP) was raised in goat using fluorescein conjugated to goat IgG as the immunogen.

Application Details

Application Notes: ELISA: 1:10,000-1:50,000, IHC: 1:500-1:2,000, WB: 1:2,000 - 1:5,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: Lot specific

Buffer: Lyophilized from 0.02 M K2 O4, pH 7.2, with 0.12 NaCl, 10 mg/mL BSA, and 0.01 % gentamycin sulfate. DO NOT add NaN3. Immunoglobulin and protease free.

Preservative: Without preservative

Handling Advice: Avoid repeated freeze/thaw cycles.
Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -20 °C for long term storage.

Target Details for FITC

Target: FITC (Fluorescein Isothiocyanate (FITC))

Alternative Name: FITC (FITC Products)

Target Type: Chemical

Background: Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins.