Streptavidin antibody (FITC)

Catalog No. ABIN7121097

Product Details anti-Streptavidin Antibody (FITC)

Target: Streptavidin

Reactivity: Streptomyces avidinii

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Streptavidin antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Purification: Ion exchange chromatography

Immunogen: Streptavidin antibody (FITC) was raised in rabbit using streptavidin as the immunogen.

Application Details

Application Notes: FC: 1:2,000-1:10,000, IF: 1:500-1:2,500, OTHER: FC: 1:2,000-1:10,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: IgG fraction supplied as a liquid in 20 mM Potassium Phosphate, 150 mM Sodium Chloride, pH 7.2, with 10 mg/mL BSA (IgG and Protease free) as a stabilizer, and 0.01 % NaN3 as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Product is photosensitive and should be protected from light.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C or aliquot and freeze at -20 °C for long term storage.

Target Details for Streptavidin

Target: Streptavidin

Abstract: Streptavidin Products

Background: Streptavidin is a 52,800 dalton tetrameric protein purified from the bacterium Streptomyces avidinii. It has an extraordinarily high affinity for biotin (also known as vitamin B7), the dissociation constant (kDa) of the biotin-streptavidin complex is on the order of 10-14 mol/L, making it one of the strongest non-covalent interactions known in nature. It is used extensively in molecular biology and bionanotechnology as, in addition to the high affinity, biotin-binding is resistant to extremes of pH, temperature, organic solvents, denaturants, detergents and proteolytic enzymes.