Choline Acetyltransferase antibody (N-Term)
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- Target See all Choline Acetyltransferase (CHAT) Antibodies
- Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))
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Binding Specificity
- N-Term
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Choline Acetyltransferase antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC)
- Specificity
- Predicted molecular weight at ~82.5 kDa. Observed molecular weights between 68-70 kDa.
- Cross-Reactivity
- Human, Mouse
- Purification
- Peptide Affinity Purified
- Immunogen
- Synthetic peptide from the N-terminal to the mid-protein of human Choline O-Acetyltrasferase
- Top Product
- Discover our top product CHAT Primary Antibody
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- Application Notes
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- WB (1:1000)
- ICC/IF (1:100)
- optimal dilutions for assays should be determined by the user.
- Comment
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A 1:1000 dilution of ABIN2869094 was sufficient for detection of Choline Acetyltransferase on mouse brain lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- PBS, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- -20°C
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- Target
- Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))
- Alternative Name
- Choline Acetyltransferase (CHAT Products)
- Synonyms
- CHOACTASE antibody, CMS1A antibody, CMS1A2 antibody, ChoAcTase antibody, B230380D24Rik antibody, choline O-acetyltransferase antibody, choline O-acetyltransferase a antibody, choline acetyltransferase antibody, carnitine O-acetyltransferase antibody, CHAT antibody, chata antibody, Chat antibody, PGTG_00551 antibody, LOC100533322 antibody
- Background
- Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker. ChAT is a valuable marker for diseases associated with decreased cholinergic function such as Schizophrenia, Alzheimer disease and Down syndrome (1-3).
- Gene ID
- 1103
- NCBI Accession
- NP_001136401
- UniProt
- P28329
- Pathways
- Skeletal Muscle Fiber Development
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