VRN2 antibody
VRN2
Reactivity: Wheat
ELISA, WB
Host: Mouse
Monoclonal
unconjugated
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- Target See all VRN2 products
- VRN2 (Polycomb Group Protein VERNALIZATION 2 (VRN2))
- Reactivity
- Wheat
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- ELISA, Western Blotting (WB)
- Cross-Reactivity (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
- Predicted Reactivity
- Triticum monococcum
- Characteristics
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Expected / apparent Molecular Weight of the Antigene: 23.7
VRN-2 and ZCCT2 full-length proteins were expressed as GST fusion proteins in E. coli and purified through GST sepharose columns. The purified VRN-2 andZCCT2 proteins were used to test the specificity of the VRN-2 antibody by Western blot analysis. A Western blot experiment showed that the VRN-2antibody was able to differentiate VRN-2 from the ZCCT2 protein. - Purification
- affinity purified
- Immunogen
- KLH-conjugated synthetic peptide chosen from Triticum aestivum VRN2 sequence
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- Application Notes
- 1: 1000 (WB), 1: 10 000 (ELISA)
- Comment
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there is some non-specific binding in a western blot when using anti-TaVRN2 antibody on nuclear extracts of T. monococcum
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Handling Advice
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - Storage
- -20 °C
- Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- Target
- VRN2 (Polycomb Group Protein VERNALIZATION 2 (VRN2))
- Alternative Name
- VRN2 (VRN2 Products)
- Synonyms
- REDUCED VERNALIZATION RESPONSE 2 antibody, VEFS-Box of polycomb protein antibody, VRN2 antibody
- Background
- Plants with a winter growth habit flower earlier when exposed for several weeks to cold temperatures, a process called vernalization. The wheat vernalization gene VRN-2 is a dominant repressor of flowering that is down-regulated by vernalization. Loss of function of VRN-2, whether by natural mutations or deletions, results in spring lines, which do not require vernalization to flower.
- Molecular Weight
- 23.7
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