Phospholamban antibody (phosphospecific)

Catalog No. ABIN285884

Product Details anti-Phospholamban Antibody

Target: Phospholamban (PLN)

Binding Specificity: AA 14-25, phosphospecific

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholamban antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Cow (Bovine), Dog (Canine), Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Bovine, Predicted to cross-react with human, mouse, rat, rabbit, pig and dog.1 mg/ml

Purification: Protein A affinity chromatography

Immunogen: Phospho-Phospholamban antibody was raised in rabbit using a synthetic peptide conjugated to KLH, corresponding to amino acids 14-25 of human cardiac phospholamban (RA[pS]TIEMPQQAR-C) as the immunogen.

Isotype: IgG

Application Details

Application Notes: Optimal conditions should be determined byt he investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 100 mM Tris-glycine, pH 7.4, with 150 M NaCl, 0.05 % NaN3 and 30 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

References for anti-Phospholamban antibody (ABIN285884)

Kushnir, Santulli, Reiken, Coromilas, Godfrey, Brunjes, Colombo, Yuzefpolskaya, Sokol, Kitsis, Marks: "Ryanodine Receptor Calcium Leak in Circulating B-Lymphocytes as a Biomarker in Heart Failure." in: Circulation, Vol. 138, Issue 11, pp. 1144-1154, (2019) (PubMed).

Target Details for Phospholamban

Target: Phospholamban (PLN)

Alternative Name: Phospholamban (PLN Products)

Synonyms: LOC100226261 antibody, plb antibody, cmd1p antibody, CMD1P antibody, CMH18 antibody, PLB antibody, Plb antibody, Plm antibody, phospholamban antibody, PLN antibody, pln antibody, Pln antibody

Background: Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. Following adrenergic stimulation of cardiac muscle, PLB is phosphorylated at Ser16 and at Thr17 which is correlated with stimulation of calcium transport activity across the SR membrane and relaxation of cardiac fibers.

Pathways: Negative Regulation of Transporter Activity