Bcl-2 antibody (N-Term)

Catalog No. ABIN2857047

Product Details anti-Bcl-2 Antibody

Key Features:

• High quality Bcl-2 Primary Antibody for the detection of BCL2.
• Reliable product with high standard validation data

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Bcl-2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity: Cat, Dog, Human, Mouse, Rat, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to BCL2 (B-cell CLL/lymphoma 2)
Bcl-2 antibody [N1N2], N-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Bcl-2. The exact sequence is proprietary.

Isotype: IgG

Product Specific Information:

What can the Bcl-2 Antibody ABIN2857047 be used for?: This polyclonal Bcl-2 Antibody detects Bcl-2. The Bcl-2 Antibody has been validated for various applications and can be used for the detection of Bcl-2 and derivatives by Western Blotting, Immunofluorescence, Immunohistochemistry (Paraffin-embedded Sections), Immunocytochemistry.

What validation data is available for this Bcl-2 Antibody?: The primary antibody is referenced in 13 publications and is characterized by a proven, very high reliability. It has currently 16 product images that show its performance in a variety of applications. The product is currently available in 100 μL quantity. Bcl-2 Antibody for the detection of Bcl-2 and derivatives.

What is the function of Bcl-2?: This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. [provided by RefSeq, Jul 2008].

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: BCL2-transfected 293T

Validation: Comparison, Orthogonal, Overexpression

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-Bcl-2 antibody (ABIN2857047)

Tvrdá, Ďuračka, Benko, Kováčik, Lovíšek, Gálová, Žiarovská, Schwarzová, Kačániová: "Ejaculatory Abstinence Affects the Sperm Quality in Normozoospermic Men-How Does the Seminal Bacteriome Respond?" in: International journal of molecular sciences, Vol. 24, Issue 4, (2023) (PubMed).

Lin, Lecca, Yang, Luo, Scerba, Tweedie, Huang, Jung, Kim, Yang, Hoffer, Wang, Greig: "3,6'-dithiopomalidomide reduces neural loss, inflammation, behavioral deficits in brain injury and microglial activation." in: eLife, Vol. 9, (2020) (PubMed).

Luo, Huang, Yuan, Yang, Wang, Huang, Tang, Sun: "Abdominal paracentesis drainage attenuates severe acute pancreatitis by enhancing cell apoptosis via PI3K/AKT signaling pathway." in: Apoptosis : an international journal on programmed cell death, Vol. 25, Issue 3-4, pp. 290-303, (2020) (PubMed).

Yang, Tang, Xu, Liu, Tan, Gao, Sun, Ding, Liu, Chen: "PI3K/mTORC1/2 inhibitor PQR309 inhibits proliferation and induces apoptosis in human glioblastoma cells." in: Oncology reports, Vol. 43, Issue 3, pp. 773-782, (2020) (PubMed).

Wen, Sun, Tan, Liu, Huang, Chen, Qi, Tang: "Abdominal paracentesis drainage ameliorates myocardial injury in severe experimental pancreatitis rats through suppressing oxidative stress." in: World journal of gastroenterology, Vol. 26, Issue 1, pp. 35-54, (2020) (PubMed).

Wu, Luo, Liu, Cui, Guo, Zhao, Guo, Bai, Guo, Feng, Qu et al.: "Recombinant adiponectin peptide promotes neuronal survival after intracerebral haemorrhage by suppressing mitochondrial and ATF4-CHOP apoptosis pathways in diabetic mice via Smad3 signalling ..." in: Cell proliferation, Vol. 53, Issue 2, pp. e12759, (2020) (PubMed).

Jiao, Li, Shao, Zhang, Gong, Yang, Wang, Tan, Sun, Xuan, Yu, Li, Gao, Liu, Xu, Li, Zhang, Zhang: "lncRNA-ZFAS1 induces mitochondria-mediated apoptosis by causing cytosolic Ca2+ overload in myocardial infarction mice model." in: Cell death & disease, Vol. 10, Issue 12, pp. 942, (2020) (PubMed).

Lam, Liang, Jiang, Peng: "Albendazole-Schisandrin B Co-Therapy on Angiostrongylus cantonensis-Induced Meningoencephalitis in Mice." in: Biomolecules, Vol. 10, Issue 7, (2020) (PubMed).

Wen, Liu, Lin, Luo, Tang, Huang, Sun, Tang: "NADPH Oxidase Hyperactivity Contributes to Cardiac Dysfunction and Apoptosis in Rats with Severe Experimental Pancreatitis through ROS-Mediated MAPK Signaling Pathway." in: Oxidative medicine and cellular longevity, Vol. 2019, pp. 4578175, (2019) (PubMed).

Batsaikhan, Wang, Scerba, Tweedie, Greig, Miller, Hoffer, Lin, Wang: "Post-Injury Neuroprotective Effects of the Thalidomide Analog 3,6'-Dithiothalidomide on Traumatic Brain Injury." in: International journal of molecular sciences, Vol. 20, Issue 3, (2019) (PubMed).

Xiao, Zeng, Guan, Wang, Wang, Shi, Shou, Deng, Xin: "FKBP12.6 protects heart from AngII-induced hypertrophy through inhibiting Ca2+ /calmodulin-mediated signalling pathways in vivo and in vitro." in: Journal of cellular and molecular medicine, Vol. 22, Issue 7, pp. 3638-3651, (2019) (PubMed).

Li, Luo, Dong, Liu, Wu, Zhang, Hu, Zhang, Song, Li: "Therapeutic effect of urine-derived stem cells for protamine/lipopolysaccharide-induced interstitial cystitis in a rat model." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 107, (2018) (PubMed).

Wang, Hsieh, Liu, Lin, Wu, Chen, Fang: "Reactive oxygen species-driven mitochondrial injury induces apoptosis by teroxirone in human non-small cell lung cancer cells." in: Oncology letters, Vol. 14, Issue 3, pp. 3503-3509, (2017) (PubMed).

Target Details for Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Alternative Name: BCL2 apoptosis regulator (BCL2 Products)

Synonyms: Bcl-2 antibody, PPP1R50 antibody, bcl-2 antibody, AW986256 antibody, C430015F12Rik antibody, D630044D05Rik antibody, D830018M01Rik antibody, BCL-2 antibody, Bcl2 antibody, BCL2, apoptosis regulator antibody, B cell leukemia/lymphoma 2 antibody, B-cell CLL/lymphoma 2a antibody, B-cell CLL/lymphoma 2 protein antibody, BCL2 antibody, Bcl2 antibody, bcl2a antibody, BCL-2 antibody

Background: This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends.

Cellular Localization: Mitochondrion outer membrane , Nucleus membrane , Endoplasmic reticulum membrane

Molecular Weight: 26 kDa

Gene ID: 596

UniProt: P10415

Pathways: MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling