RORA antibody

Catalog No. ABIN2857022

Product Details anti-RORA Antibody

Target: RORA (RAR-Related Orphan Receptor A (RORA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RORA antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to ROR alpha (RAR-related orphan receptor A)
ROR alpha antibody [N2N3]

Purification: Purified by antigen-affinity chromatography.

Grade: KO Validated

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human ROR alpha. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , HeLa , NIH-3T3

Validation: KO/KD

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-RORA antibody (ABIN2857022)

Luo, Wang, Yeh, Sun, Liang, Xiao, Xu, Niu, Cheng, Maity, Jiang, Chang: "LncRNA-p21 alters the antiandrogen enzalutamide-induced prostate cancer neuroendocrine differentiation via modulating the EZH2/STAT3 signaling." in: Nature communications, Vol. 10, Issue 1, pp. 2571, (2019) (PubMed).

Brożyna, Jóźwicki, Skobowiat, Jetten, Slominski: "RORα and RORγ expression inversely correlates with human melanoma progression." in: Oncotarget, Vol. 7, Issue 39, pp. 63261-63282, (2018) (PubMed).

Target Details for RORA

Target: RORA (RAR-Related Orphan Receptor A (RORA))

Alternative Name: RAR related orphan receptor A (RORA Products)

Background: The protein encoded by this gene is a member of the NR1 subfamily of nuclear hormone receptors. It can bind as a monomer or as a homodimer to hormone response elements upstream of several genes to enhance the expression of those genes. The specific functions of this protein are not known, but it has been shown to interact with NM23-2, a nucleoside diphosphate kinase involved in organogenesis and differentiation, as well as with NM23-1, the product of a tumor metastasis suppressor candidate gene. Four transcript variants encoding different isoforms have been described for this gene.

Molecular Weight: 63 kDa

Gene ID: 6095

UniProt: P35398

Pathways: Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Lipid Metabolism by PPARalpha