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HRAS antibody (C-Term)

Name: HRAS antibody (C-Term)
Brand: antibodies-online
SKU: ABIN2857002
Price: 609.18 USD
Availability: InStock

HRAS Reactivity: Human, Mouse, Rat WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

WB Image Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with H-Ras antibody , diluted at 1:5000. (HRAS antibody (C-Term))

IHC-P Image H-Ras antibody detects H-Ras protein at membrane on mouse intestine by immunohistochemical analysis. (HRAS antibody (C-Term))

IHC

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using H-Ras , antibody at 1:200 dilution. (HRAS antibody (C-Term))

WB Image Sample (30 ug of whole cell lysate) A: Hela B: Hep G2 , C: Molt-4 , 12% SDS PAGE antibody diluted at 1:1000 (HRAS antibody (C-Term))

IHC-P Image H-Ras antibody detects H-Ras protein at membrane on mouse prostate by immunohistochemical analysis. (HRAS antibody (C-Term))

IHC

WB Image H-Ras antibody detects HRAS protein by Western blot analysis. (HRAS antibody (C-Term))

mRNA expression (all n = 6) of HRas, MAPK14 (p38), CCL2, DOK1 and PTK2B related to GAPDH mRNA expression, protein expression of HRas (n = 6), p38 (n = 6), CCL2 (n = 4), DOK1 (n = 7-8) and PTK2B (n = 3) related to GAPDH expression in A549 () and A549rCDDP2000 () before (ctrl) and after treatment with 11 μM cisplatin (11) or 34 μM cisplatin (34) presented as mean ± SEM, as well as representative Western blots. (HRAS antibody (C-Term))

Overexpression of Nogo-B receptor (NgBR) increases the resistance of MCF-7 cells to tamoxifen. (HRAS antibody (C-Term))

8 images

(4 references)

Catalog No. ABIN2857002

Datasheet as PDF

Target See all HRAS Antibodies

HRAS (HRas proto-oncogene, GTPase (HRAS))
Binding Specificity
15

15

13

12

7

6

5

3

3

2

2

2

2

2

1

1

1

1
C-Term
Reactivity
71

55

40

7

4

3

2

2

1

1

1

1

1

1

1
Human, Mouse, Rat
Host
93

10

1
Rabbit
Compatible Secondaries
Clonality
93

11
Polyclonal
Conjugate
40

11

10

6

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
This HRAS antibody is un-conjugated
Application
89

43

26

26

15

14

10

9

7

3

2

1

1

1

1

1

1
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Characteristics

Rabbit Polyclonal antibody to H-Ras (v-Ha-ras Harvey rat sarcoma viral oncogene homolog)
H-Ras antibody

Purification

Purified by antigen-affinity chromatography.

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human H-Ras.
anti-HRas proto-oncogene, GTPase (HRAS) (AA 104-128) antibody
HRAS Reactivity: Human WB, IF, IHC (p) Host: Rabbit Polyclonal RB15622 unconjugated

anti-HRas proto-oncogene, GTPase (HRAS) antibody
HRAS Reactivity: Human, Mouse, Rat, Dog, Monkey WB, IHC, IF, IHC (p) Host: Mouse Monoclonal 1C2 unconjugated

anti-HRas proto-oncogene, GTPase (HRAS) (AA 104-128) antibody
HRAS Reactivity: Human WB, ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-HRas proto-oncogene, GTPase (HRAS) antibody
HRAS Reactivity: Human, Mouse, Rat, Dog, Monkey WB, IHC, IF, IHC (p) Host: Mouse Monoclonal 1A1 unconjugated

anti-HRas proto-oncogene, GTPase (HRAS) (AA 146-176) antibody
HRAS Reactivity: Human, Mouse WB, ELISA, IF Host: Rabbit Polyclonal unconjugated

anti-HRas proto-oncogene, GTPase (HRAS) (AA 146-176), (C-Term) antibody
HRAS Reactivity: Human, Mouse WB, IF Host: Rabbit Polyclonal RB14565 unconjugated

anti-HRas proto-oncogene, GTPase (HRAS) (AA 2-186) antibody
HRAS Reactivity: Rat WB, ELISA, IHC Host: Rabbit Polyclonal unconjugated

anti-HRas proto-oncogene, GTPase (HRAS) (AA 12-181) antibody
HRAS Reactivity: Human ELISA, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-HRas proto-oncogene, GTPase (HRAS) antibody
HRAS Reactivity: Human WB Host: Mouse Monoclonal H-RAS-03 unconjugated

anti-HRas proto-oncogene, GTPase (HRAS) (AA 1-189) antibody
HRAS Reactivity: Human WB, PLA Host: Mouse Polyclonal unconjugated

Application Notes

WB: 1:500-1:3000, IHC-P: 1:100-1:1000, ICC/IF: 1:100-1:1000.
Not yet tested in other applications.
Optimal working dilutions should be determined experimentally by the end user.

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

0.1M Tris, 0.1M Glycine, 20 % Glycerol ( pH 7).
0.01 % Thimerosal was added as a preservative.

Preservative

Thimerosal (Merthiolate)

Precaution of Use

This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

-20 °C

Storage Comment

Keep as concentrated solution. Aliquot and store at -20°C or below.
Avoid multiple freeze-thaw cycles.
Basu, Lee, Salotti, Basu, Sakchaisri, Xiao, Walia, Westlake, Morrison, Johnson: "Oncogenic RAS-Induced Perinuclear Signaling Complexes Requiring KSR1 Regulate Signal Transmission to Downstream Targets." in: Cancer research, Vol. 78, Issue 4, pp. 891-908, (2019) (PubMed).

Brandt, Albers, Hejhal, Pfundstein, Gonçalves, Catalano, Wild, Frew: "Mouse genetic background influences whether HrasG12V expression plus Cdkn2a knockdown causes angiosarcoma or undifferentiated pleomorphic sarcoma." in: Oncotarget, Vol. 9, Issue 28, pp. 19753-19766, (2018) (PubMed).

Gao, Wang, Jin, Hu, Duan, Shi, Du, Song, Yang, Li, Han, Zhao, Zhang, Fan, Miao: "Nogo-B receptor increases the resistance to tamoxifen in estrogen receptor-positive breast cancer cells." in: Breast cancer research : BCR, Vol. 20, Issue 1, pp. 112, (2018) (PubMed).

Brandt, Albers, Hejhal, Catalano, Wild, Frew: "Oncogenic HrasG12V expression plus knockdown of Cdkn2a using ecotropic lentiviral vectors induces high-grade endometrial stromal sarcoma." in: PLoS ONE, Vol. 12, Issue 10, pp. e0186102, (2017) (PubMed).
Target

HRAS (HRas proto-oncogene, GTPase (HRAS))

Alternative Name

H-Ras (HRAS Products)

Synonyms

C-BAS/HAS antibody, C-H-RAS antibody, C-HA-RAS1 antibody, CTLO antibody, H-RASIDX antibody, HAMSV antibody, HRAS1 antibody, K-RAS antibody, N-RAS antibody, RASH1 antibody, hras antibody, zgc:110250 antibody, HRAS antibody, H-RAS antibody, c-H-ras antibody, H-Ras antibody, K-Ras antibody, hras1 antibody, rash1 antibody, ras antibody, N-Ras antibody, c-bas/has antibody, H-ras antibody, Ha-ras antibody, Harvey-ras antibody, Hras-1 antibody, Kras2 antibody, c-Ha-ras antibody, c-rasHa antibody, hrasl antibody, zgc:110734 antibody, HRas proto-oncogene, GTPase antibody, v-Ha-ras Harvey rat sarcoma viral oncogene homolog a antibody, neuroblastoma RAS viral (v-ras) oncogene homolog pseudogene antibody, Harvey rat sarcoma viral oncogene homolog L homeolog antibody, Harvey rat sarcoma viral oncogene homolog antibody, Harvey rat sarcoma virus oncogene antibody, NRAS proto-oncogene, GTPase antibody, -Ha-ras Harvey rat sarcoma viral oncogene homolog b antibody, HRAS antibody, hrasa antibody, LOC733587 antibody, Hras antibody, hras.L antibody, hras antibody, NRAS antibody, hrasb antibody

Gene ID

3265

Pathways

p53 Signaling, MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hepatitis C, Autophagy, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Regulation of long-term Neuronal Synaptic Plasticity, VEGF Signaling, BCR Signaling