HOMER3
Reactivity: Human, Rat, Dog, Horse, Cow, Pig, Bat, Monkey
WB, IHC, IHC (p)
Host: Rabbit
Polyclonal
unconjugated
Application Notes
WB: 1:500-1:3000, ICC/IF: 1:100-1:1000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer ( pH 6.0) or Tris-EDTA buffer ( pH 8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
0.1M Tris, 0.1M Glycine, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative
Thimerosal (Merthiolate)
Precaution of Use
This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Chen, Zhang, Wang, Zeng, Li, Li, Li, You, Zhang, Tzeng: "WW domain-binding protein 2 acts as an oncogene by modulating the activity of the glycolytic enzyme ENO1 in glioma." in: Cell death & disease, Vol. 9, Issue 3, pp. 347, (2019) (PubMed).