WB: 1:5000-1:20000, IHC-P: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Restrictions
For Research Use only
Format
Liquid
Concentration
0.96 mg/mL
Buffer
0.1M Tris, 0.1M Glycine, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative
Thimerosal (Merthiolate)
Precaution of Use
This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Lin, Kiihl, Suhail, Liu, Chou, Kuang, Lu, Khor, Lin, Bader, Irizarry, Boeke: "Functional dissection of lysine deacetylases reveals that HDAC1 and p300 regulate AMPK." in: Nature, Vol. 482, Issue 7384, pp. 251-5, (2012) (PubMed).