VCP antibody (N-Term)

Catalog No. ABIN2856882

Product Details anti-VCP Antibody

Target: VCP (Valosin Containing Protein (VCP))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VCP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Whole Mount) (IHC (wm))

Cross-Reactivity: Dog, Human, Mouse, Rat, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to VCP (valosin-containing protein)
VCP antibody [N1N2], N-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human VCP. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , rat brain , A549 , H1299 , HCT116

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-VCP antibody (ABIN2856882)

Tsuji, Ohama, Nakagawa, Sato: "Efficacy of an anti-cancer strategy targeting SET in canine osteosarcoma." in: The Journal of veterinary medical science, Vol. 81, Issue 10, pp. 1424-1430, (2020) (PubMed).

Kake, Tsuji, Enjoji, Hanasaki, Hayase, Yabe, Tanaka, Nakagawa, Liu, Chang, Usui, Ohama, Sato: "The role of SET/I2PP2A in canine mammary tumors." in: Scientific reports, Vol. 7, Issue 1, pp. 4279, (2019) (PubMed).

Yabe, Tsuji, Mochida, Ikehara, Usui, Ohama, Sato: "A stable association with PME-1 may be dispensable for PP2A demethylation - implications for the detection of PP2A methylation and immunoprecipitation." in: FEBS open bio, Vol. 8, Issue 9, pp. 1486-1496, (2018) (PubMed).

Target Details for VCP

Target: VCP (Valosin Containing Protein (VCP))

Alternative Name: valosin containing protein (VCP Products)

Synonyms: ALS14 antibody, IBMPFD antibody, TERA antibody, p97 antibody, CDC48 antibody, wu:fd16d05 antibody, wu:fj63d11 antibody, p93 antibody, 3110001E05 antibody, p97/VCP antibody, 22.26 antibody, 7.12 antibody, Apple antibody, BcDNA.GM02885 antibody, BcDNA:GM02885 antibody, CG2331 antibody, DmTER94 antibody, Dmel\CG2331 antibody, I antibody, Ter94 antibody, Tera antibody, VCP antibody, VCP ATPase antibody, VCP/p97 antibody, Vcp antibody, anon-WO2004063362.65 antibody, anon-WO2004063362.67 antibody, cdc48 antibody, dTERA antibody, dVCP antibody, dmTERA antibody, dvcp antibody, l(2)03775 antibody, l(2)46CFf antibody, l(2)46CFs antibody, l(2)46Ch antibody, pTER94 antibody, ter94 antibody, vcp antibody, FANCG antibody, valosin containing protein antibody, valosin containing protein S homeolog antibody, valosin-containing protein antibody, CG2331 gene product from transcript CG2331-RA antibody, zgc:136908 antibody, transitional endoplasmic reticulum ATPase antibody, VCP antibody, vcp.S antibody, vcp antibody, Vcp antibody, Bm1_56585 antibody, LOC100136246 antibody, TER94 antibody, zgc:136908 antibody, LOC101109680 antibody

Molecular Weight: 89 kDa

Gene ID: 7415

UniProt: P55072

Pathways: ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway