VCP antibody (N-Term)

Catalog No. ABIN2856882

Product Details anti-VCP Antibody

Target: VCP (Valosin Containing Protein (VCP))

Binding Specificity: N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VCP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Whole Mount) (IHC (wm))

Cross-Reactivity: Dog, Human, Mouse, Rat, Zebrafish (Danio rerio)

Characteristics: Rabbit Polyclonal antibody to VCP (valosin-containing protein)
VCP antibody [N1N2], N-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human VCP. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:1000-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: Mouse brain , rat brain , A549 , H1299 , HCT116

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/mL

Buffer: 1XPBS ( pH 7), 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-VCP antibody (ABIN2856882)

Tsuji, Ohama, Nakagawa, Sato: "Efficacy of an anti-cancer strategy targeting SET in canine osteosarcoma." in: The Journal of veterinary medical science, Vol. 81, Issue 10, pp. 1424-1430, (2020) (PubMed).

Kake, Tsuji, Enjoji, Hanasaki, Hayase, Yabe, Tanaka, Nakagawa, Liu, Chang, Usui, Ohama, Sato: "The role of SET/I2PP2A in canine mammary tumors." in: Scientific reports, Vol. 7, Issue 1, pp. 4279, (2019) (PubMed).

Yabe, Tsuji, Mochida, Ikehara, Usui, Ohama, Sato: "A stable association with PME-1 may be dispensable for PP2A demethylation - implications for the detection of PP2A methylation and immunoprecipitation." in: FEBS open bio, Vol. 8, Issue 9, pp. 1486-1496, (2018) (PubMed).

Target Details for VCP

Target: VCP (Valosin Containing Protein (VCP))

Alternative Name: valosin containing protein (VCP Products)

Molecular Weight: 89 kDa

Gene ID: 7415

UniProt: P55072

Pathways: ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway