DTX1
Reactivity: Mouse
ELISA, WB, IF (cc), IF (p), IHC (p), IHC (fro), ICC
Host: Rabbit
Polyclonal
unconjugated
Application Notes
WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10 mM Citrate buffer ( pH 6.0) or Tris-EDTA buffer ( pH 8.0)], ICC/IF: 1:100-1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
0.1M Tris, 0.1M Glycine, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative
Thimerosal (Merthiolate)
Precaution of Use
This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C
Storage Comment
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Hou, Zhao, Zhang, Wang, Ye, Wang, Ren, Zhang, Lu: "LSD1 regulates Notch and PI3K/Akt/mTOR pathways through binding the promoter regions of Notch target genes in esophageal squamous cell carcinoma." in: OncoTargets and therapy, Vol. 12, pp. 5215-5225, (2019) (PubMed).