Cytokeratin 8/18 antibody

Catalog No. ABIN285686

Product Details anti-Cytokeratin 8/18 Antibody

Target: Cytokeratin 8/18

Reactivity: Cow

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This Cytokeratin 8/18 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), ELISA

Purity: Stabilized antiserum

Immunogen: Cytokeratin 8/8 antibody was raised in guinea pig using cytokeratin 8/18 filaments, reconstituted from purified bovine cytokeratins 8 and 18 as the immunogen.

Isotype: IgG

Application Details

Application Notes: IHC-F: 1:100, IHC-P: 1:50, WB: 1:2,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as whole antiserum with 0.09 % NaN3.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide as preservative. Although the amount of sodium azide is very small appropriate care must be taken when handling this product.

Storage: 4 °C

Storage Comment: Store at 4 °C

References for anti-Cytokeratin 8/18 antibody (ABIN285686)

Ruetten, Cole, Wehber, Wegner, Girardi, Peterson, Scharpf, Romero, Wood, Colopy, Bjorling, Vezina: "An immunohistochemical prostate cell identification key indicates that aging shifts procollagen 1A1 production from myofibroblasts to fibroblasts in dogs prone to prostate-related urinary dysfunction." in: PLoS ONE, Vol. 15, Issue 7, pp. e0232564, (2020) (PubMed).

Xiao, Zuo, Hirukawa, Cardiff, Lamb, Sonenberg, Muller: "Rheb1-Independent Activation of mTORC1 in Mammary Tumors Occurs through Activating Mutations in mTOR." in: Cell reports, Vol. 31, Issue 4, pp. 107571, (2020) (PubMed).

Pfefferle, Darr, Calhoun, Mott, Rosen, Perou: "The MMTV-Wnt1 murine model produces two phenotypically distinct subtypes of mammary tumors with unique therapeutic responses to an EGFR inhibitor." in: Disease models & mechanisms, Vol. 12, Issue 7, (2020) (PubMed).

Pénzváltó, Chen, Tepper, Davis, Silvestrini, Umeh-Garcia, Sweeney, Borowsky: "A Syngeneic ErbB2 Mammary Cancer Model for Preclinical Immunotherapy Trials." in: Journal of mammary gland biology and neoplasia, Vol. 24, Issue 2, pp. 149-162, (2020) (PubMed).

Hirukawa, Smith, Zuo, Dufour, Savage, Bertos, Johnson, Bui, Bourque, Basik, Giguère, Park, Muller: "Targeting EZH2 reactivates a breast cancer subtype-specific anti-metastatic transcriptional program." in: Nature communications, Vol. 9, Issue 1, pp. 2547, (2018) (PubMed).

Farrell, Joly, Allen-Petersen, Worth, Lanciault, Sauer, Link, Pelz, Heiser, Morton, Muthalagu, Hoffman, Manning, Pratt, Kendsersky, Egbukichi, Amery, Thoma, Jenny, Rhim, Murphy, Sansom, Crawford et al.: "MYC regulates ductal-neuroendocrine lineage plasticity in pancreatic ductal adenocarcinoma associated with poor outcome and chemoresistance. ..." in: Nature communications, Vol. 8, Issue 1, pp. 1728, (2018) (PubMed).

Mekhdjian, Kai, Rubashkin, Prahl, Przybyla, McGregor, Bell, Barnes, DuFort, Ou, Chang, Cassereau, Tan, Pickup, Lakins, Ye, Davidson, Lammerding, Odde, Dunn, Weaver: "Integrin-mediated traction force enhances paxillin molecular associations and adhesion dynamics that increase the invasiveness of tumor cells into a three-dimensional extracellular matrix." in: Molecular biology of the cell, Vol. 28, Issue 11, pp. 1467-1488, (2017) (PubMed).

Du, Chen, Cao, Lu, Zhang, Bornstein, Li, Owens, Malkoski, Said, Jin, Kulesz-Martin, Gross, Wang, Lu: "Overexpression of PIK3CA in murine head and neck epithelium drives tumor invasion and metastasis through PDK1 and enhanced TGFβ signaling." in: Oncogene, Vol. 35, Issue 35, pp. 4641-52, (2017) (PubMed).

Britschgi, Duss, Kim, Couto, Brinkhaus, Koren, De Silva, Mertz, Kaup, Varga, Voshol, Vissieres, Leroy, Roloff, Stadler, Scheel, Miraglia, Orth, Bonamy, Reddy, Bentires-Alj: "The Hippo kinases LATS1 and 2 control human breast cell fate via crosstalk with ERα." in: Nature, Vol. 541, Issue 7638, pp. 541-545, (2017) (PubMed).

Pickup, Hover, Guo, Gorska, Chytil, Novitskiy, Moses, Owens: "Deletion of the BMP receptor BMPR1a impairs mammary tumor formation and metastasis." in: Oncotarget, Vol. 6, Issue 26, pp. 22890-904, (2016) (PubMed).

Owens, Pickup, Novitskiy, Giltnane, Gorska, Hopkins, Hong, Moses: "Inhibition of BMP signaling suppresses metastasis in mammary cancer." in: Oncogene, Vol. 34, Issue 19, pp. 2437-49, (2015) (PubMed).

Matise, Palmer, Ashby, Nashabi, Chytil, Aakre, Pickup, Gorska, Zijlstra, Moses: "Lack of transforming growth factor-β signaling promotes collective cancer cell invasion through tumor-stromal crosstalk." in: Breast cancer research : BCR, Vol. 14, Issue 4, pp. R98, (2015) (PubMed).

Vaisman, Vishnyakova, Freeman, Amar, Demosky, Liu, Stonik, Sampson, Pryor, Bocharov, Eggerman, Patterson, Remaley: "Endothelial Expression of Scavenger Receptor Class B, Type I Protects against Development of Atherosclerosis in Mice." in: BioMed research international, Vol. 2015, pp. 607120, (2015) (PubMed).

Ferrari, Riggio, Mason, McDonald, King, Higgins, Rosewell, Neil, Smalley, Sansom, Morris, Cameron, Blyth: "Runx2 contributes to the regenerative potential of the mammary epithelium." in: Scientific reports, Vol. 5, pp. 15658, (2015) (PubMed).

Balko, Giltnane, Wang, Schwarz, Young, Cook, Owens, Sanders, Kuba, Sánchez, Kurupi, Moore, Pinto, Doimi, Gómez, Horiuchi, Goga, Lehmann, Bauer, Pietenpol, Ross, Palmer, Yelensky, Cronin, Miller et al.: "Molecular profiling of the residual disease of triple-negative breast cancers after neoadjuvant chemotherapy identifies actionable therapeutic targets. ..." in: Cancer discovery, Vol. 4, Issue 2, pp. 232-45, (2014) (PubMed).

McDonald, Ferrari, Terry, Bell, Mohammed, Orange, Jenkins, Muller, Gusterson, Neil, Edwards, Morris, Cameron, Blyth: "RUNX2 correlates with subtype-specific breast cancer in a human tissue microarray, and ectopic expression of Runx2 perturbs differentiation in the mouse mammary gland." in: Disease models & mechanisms, Vol. 7, Issue 5, pp. 525-34, (2014) (PubMed).

Waldmeier, Meyer-Schaller, Diepenbruck, Christofori: "Py2T murine breast cancer cells, a versatile model of TGFβ-induced EMT in vitro and in vivo." in: PLoS ONE, Vol. 7, Issue 11, pp. e48651, (2013) (PubMed).

Watson, Lai, Qin, Kruse, Lin, Seo, Cardiff, Mahakian, Beegle, Ingham, Curry, Reed, Ferrara: "Ultrasound increases nanoparticle delivery by reducing intratumoral pressure and increasing transport in epithelial and epithelial-mesenchymal transition tumors." in: Cancer research, Vol. 72, Issue 6, pp. 1485-93, (2012) (PubMed).

Target Details for Cytokeratin 8/18

Target: Cytokeratin 8/18

Alternative Name: Cytokeratin 8 +18 (Cytokeratin 8/18 Products)