CASP14 antibody (C-Term)

Catalog No. ABIN2856832

Product Details anti-CASP14 Antibody

Target: CASP14 (Caspase 14, Apoptosis-Related Cysteine Peptidase (CASP14))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CASP14 antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Human

Characteristics: Rabbit polyclonal antibody to Caspase 14 (caspase 14, apoptosis-related cysteine peptidase)
Caspase 14 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Caspase 14. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: A431 , PC-3

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-CASP14 antibody (ABIN2856832)

Dang, Pang, Song, An, Ma: "Inactivation of mitogen-activated protein kinase signaling pathway reduces caspase-14 expression in impaired keratinocytes." in: Iranian journal of basic medical sciences, Vol. 19, Issue 1, pp. 28-33, (2016) (PubMed).

Target Details for CASP14

Target: CASP14 (Caspase 14, Apoptosis-Related Cysteine Peptidase (CASP14))

Alternative Name: caspase 14 (CASP14 Products)

Synonyms: CASP14 antibody, MICE antibody, mini-ICE antibody, caspase 14, apoptosis-related cysteine peptidase antibody, caspase 14 antibody, caspase-14-like antibody, caspase-14 antibody, CASP14 antibody, Casp14 antibody, LOC485601 antibody, LOC780455 antibody

Background: This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This caspase has been shown to be processed and activated by caspase 8 and caspase 10 in vitro, and by anti-Fas agonist antibody or TNF-related apoptosis inducing ligand in vivo. The expression and processing of this caspase may be involved in keratinocyte terminal differentiation, which is important for the formation of the skin barrier.

Cellular Localization: Cytoplasm , Nucleus

Molecular Weight: 28 kDa

Gene ID: 23581

UniProt: P31944