HSP70 1A antibody (Center)

Catalog No. ABIN2856787

Product Details anti-HSP70 1A Antibody

Target: HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))

Binding Specificity: Center

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP70 1A antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Sheep (Ovine), Cow (Bovine)

Cross-Reactivity (Details): Sheep (100 %), Bovine (100 %)

Characteristics: Rabbit Polyclonal antibody to HSP70 1A (heat shock 70 kDa protein 1A)
HSP70 1A antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human HSP70 1A. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:500-1:1000* Western blot 1:5000-1:20000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:500-1:1000* Western blot1:5000-1:20000*

Comment:

Positive Control: 293T , NIH-3T3 , Mouse brain , PC-12 , Rat brain , Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , *Mesenchymal stem cells

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-HSP70 1A antibody (ABIN2856787)

Chen, Lin, Guo, Chiu, Wu, Jia, Yen: "Rrm2b deletion causes mitochondrial metabolic defects in renal tubules." in: Scientific reports, Vol. 9, Issue 1, pp. 13238, (2019) (PubMed).

Target Details for HSP70 1A

Target: HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))

Alternative Name: HSPA1A (HSPA1A Products)

Background: This intronless gene encodes a 70 kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

Molecular Weight: 70 kDa

Gene ID: 3303

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process