Fission 1 antibody (full length)

Catalog No. ABIN2856782

Product Details anti-Fission 1 Antibody

Target: Fission 1 (FIS1)

Binding Specificity: full length

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fission 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Rhesus Monkey

Cross-Reactivity (Details): Rhesus Monkey (100 %)

Characteristics: Rabbit Polyclonal antibody to FIS1 (fission 1 (mitochondrial outer membrane) homolog (S. cerevisiae))
FIS1 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Full length human FIS1 Recombinant protein.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:2000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:20000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:2000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:20000*

Comment:

Positive Control: Raji , 293T , A431 , HeLa , HepG2 , mouse liver , mouse brain , rat brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-Fission 1 antibody (ABIN2856782)

Busceti, Cotugno, Bianchi, Forte, Stanzione, Marchitti, Battaglia, Nicoletti, Fornai, Rubattu: "Brain Overexpression of Uncoupling Protein-2 (UCP2) Delays Renal Damage and Stroke Occurrence in Stroke-Prone Spontaneously Hypertensive Rats." in: International journal of molecular sciences, Vol. 21, Issue 12, (2020) (PubMed).

Ma, He, Ma, Jiang, Yan, Zhu, Bang, Li, Jia: "Silver Nanoparticle Exposure Causes Pulmonary Structural Damage and Mitochondrial Dynamic Imbalance in the Rat: Protective Effects of Sodium Selenite." in: International journal of nanomedicine, Vol. 15, pp. 633-645, (2020) (PubMed).

Lee, Chau, Ng, Chen, Wang, Liou, Lin, Chen: "Empagliflozin Protects HK-2 Cells from High Glucose-Mediated Injuries via a Mitochondrial Mechanism." in: Cells, Vol. 8, Issue 9, (2020) (PubMed).

Xian, Yang, Xiao, Shen, Liou: "STX17 dynamically regulated by Fis1 induces mitophagy via hierarchical macroautophagic mechanism." in: Nature communications, Vol. 10, Issue 1, pp. 2059, (2019) (PubMed).

Moore, Zhou, Cohn, Norheim, Lin, Kalajian, Strumwasser, Cory, Whitney, Ho, Ho, Lee, Rucker, Shirihai, van der Bliek, Whitelegge, Seldin, Lusis, Lee, Drevon, Mahata, Turcotte, Hevener: "The impact of exercise on mitochondrial dynamics and the role of Drp1 in exercise performance and training adaptations in skeletal muscle." in: Molecular metabolism, Vol. 21, pp. 51-67, (2019) (PubMed).

Bi, Zhang, Ren, Du, Li, Wang, Wei, Yang, Zhang, Liu, Lv, Wu: "Irisin alleviates liver ischemia-reperfusion injury by inhibiting excessive mitochondrial fission, promoting mitochondrial biogenesis and decreasing oxidative stress." in: Redox biology, Vol. 20, pp. 296-306, (2019) (PubMed).

Mizuno, Kuno, Yano, Miki, Oshima, Sato, Nakata, Kimura, Tanno, Miura: "Empagliflozin normalizes the size and number of mitochondria and prevents reduction in mitochondrial size after myocardial infarction in diabetic hearts." in: Physiological reports, Vol. 6, Issue 12, pp. e13741, (2019) (PubMed).

Zhang, Ma: "Mitochondrial Dynamics in Rat Heart Induced by 5-Fluorouracil." in: Medical science monitor : international medical journal of experimental and clinical research, Vol. 24, pp. 6666-6672, (2018) (PubMed).

Cheng, Kuo, Ouyang, Li, Chung, Chan, Kung, Ann: "Metabolic Stress-Induced Phosphorylation of KAP1 Ser473 Blocks Mitochondrial Fusion in Breast Cancer Cells." in: Cancer research, Vol. 76, Issue 17, pp. 5006-18, (2017) (PubMed).

Kemter, Fröhlich, Arnold, Wolf, Wanke: "Mitochondrial Dysregulation Secondary to Endoplasmic Reticulum Stress in Autosomal Dominant Tubulointerstitial Kidney Disease - UMOD (ADTKD-UMOD)." in: Scientific reports, Vol. 7, pp. 42970, (2017) (PubMed).

Yu, Liang, Li, Lee, Chang, Su, Huang, Lai, Lin: "Dengue Virus Impairs Mitochondrial Fusion by Cleaving Mitofusins." in: PLoS pathogens, Vol. 11, Issue 12, pp. e1005350, (2015) (PubMed).

Chao, Wroblewski, Ilkayeva, Stevens, Bain, Meyer, Schenk, Martinez, Vergnes, Narkar, Drew, Hong, Boyadjian, Hevener, Evans, Reue, Spencer, Newgard, Tontonoz: "Skeletal muscle Nur77 expression enhances oxidative metabolism and substrate utilization." in: Journal of lipid research, Vol. 53, Issue 12, pp. 2610-9, (2012) (PubMed).

Target Details for Fission 1

Target: Fission 1 (FIS1)

Alternative Name: FIS1 (FIS1 Products)

Synonyms: TTC11 antibody, 2010003O14Rik antibody, Ttc11 antibody, fission, mitochondrial 1 antibody, FIS1 antibody, Fis1 antibody

Background: The balance between fission and fusion regulates the morphology of mitochondria. TTC11 is a component of a mitochondrial complex that promotes mitochondrial fission (James et al., 2003 [PubMed 12783892]).[supplied by OMIM]

Cellular Localization: Mitochondrion outer membrane, Single-pass membrane protein , Peroxisome membrane, Single-pass membrane protein

Molecular Weight: 17 kDa

Gene ID: 51024

Pathways: Positive Regulation of Endopeptidase Activity