KRT20 antibody

Catalog No. ABIN2856768

Product Details anti-KRT20 Antibody

Target: KRT20 (Keratin 20 (KRT20))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KRT20 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity: Human, Mouse

Characteristics: Rabbit polyclonal antibody to Cytokeratin 20 (keratin 20)
Cytokeratin 20 antibody [N2C2], Internal

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human Cytokeratin 20. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: HeLa

Validation: Orthogonal

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.88 mg/mL

Buffer: 1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-KRT20 antibody (ABIN2856768)

Yen, Chien, Lai, Chen, Chuong, Hung, Hung: "PP2A Deficiency Enhances Carcinogenesis of Lgr5+ Intestinal Stem Cells Both in Organoids and In Vivo." in: Cells, Vol. 9, Issue 1, (2020) (PubMed).

Target Details for KRT20

Target: KRT20 (Keratin 20 (KRT20))

Alternative Name: keratin 20 (KRT20 Products)

Synonyms: KRT20 antibody, CD20 antibody, CK-20 antibody, CK20 antibody, K20 antibody, KRT21 antibody, Krt21 antibody, 9030623C06Rik antibody, keratin 20 antibody, KRT20 antibody, Krt20 antibody

Background: The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21.

Cellular Localization: Cytoplasm

Molecular Weight: 48 kDa

Gene ID: 54474

UniProt: P35900