FIG4 antibody (Center)

Catalog No. ABIN2856730

Product Details anti-FIG4 Antibody

Target: FIG4 (FIG4 Homolog, SAC1 Lipid Phosphatase Domain Containing (FIG4))

Binding Specificity: Center

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FIG4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunofluorescence (IF)

Cross-Reactivity: Chimpanzee

Cross-Reactivity (Details): Chimpanzee (100 %)

Characteristics: Rabbit Polyclonal antibody to FIG4 (FIG4 homolog, SAC1 lipid phosphatase domain containing (S. cerevisiae))
FIG4 antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human FIG4. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*

Comment:

Positive Control: HeLa , HCT116

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for FIG4

Target: FIG4 (FIG4 Homolog, SAC1 Lipid Phosphatase Domain Containing (FIG4))

Alternative Name: FIG4 (FIG4 Products)

Synonyms: A530089I17Rik antibody, AI326867 antibody, Sac3 antibody, ALS11 antibody, CMT4J antibody, KIAA0274 antibody, SAC3 antibody, YVS antibody, dJ249I4.1 antibody, Kiaa0274 antibody, RGD1311375 antibody, FIG4 phosphoinositide 5-phosphatase antibody, Fig4 antibody, FIG4 antibody

Background: The protein encoded by this gene belongs to the SAC domain-containing protein gene family. The SAC domain, approximately 400 amino acids in length and consisting of seven conserved motifs, has been shown to possess phosphoinositide phosphatase activity. The yeast homolog, Sac1p, is involved in the regulation of various phosphoinositides, and affects diverse cellular functions such as actin cytoskeleton organization, Golgi function, and maintenance of vacuole morphology. Membrane-bound phosphoinositides function as signaling molecules and play a key role in vesicle trafficking in eukaryotic cells. Mutations in this gene have been associated with Charcot-Marie-Tooth disease, type 4J.

Cellular Localization: Endosome membrane

Molecular Weight: 104 kDa

Gene ID: 9896

Pathways: Inositol Metabolic Process