BrdU antibody

Catalog No. ABIN285672

Product Details anti-BrdU Antibody

Target: BrdU (Bromodeoxyuridine (BrdU))

Reactivity: Please inquire

Host: Sheep

Clonality: Polyclonal

Conjugate: This BrdU antibody is un-conjugated

Application: Please inquire

Cross-Reactivity (Details): Not species specific. Nucleotide is present in all species. Very low cross reactivity to 5-methyl cytosine.

Purification: purified

Immunogen: BRDU antibody was raised in sheep using highly pure bromodeoxyuridine as the immunogen.

Application Details

Application Notes: Optimal conditions should be determined byt he investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: Protein G purified and supplied in PBS buffer, with no preservative.

Preservative: Without preservative

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: -20 °C/-80 °C

Storage Comment: Aliquot and store at -20 °C for short term storage, -70 °C for long-term storage.

References for anti-BrdU antibody (ABIN285672)

Ghosh, Lau, Simons, Powell, Meyers, De Marzo, Berman, Lotan: "PI3K/mTOR signaling regulates prostatic branching morphogenesis." in: Developmental biology, Vol. 360, Issue 2, pp. 329-42, (2011) (PubMed).

Ashley, Grace, Vanpoucke, Thomson: "Identification of EphrinB1 expression in prostatic mesenchyme and a role for EphB-EphrinB signalling in prostate development." in: Differentiation; research in biological diversity, Vol. 80, Issue 2-3, pp. 89-98, (2010) (PubMed).

Tavazoie, Van der Veken, Silva-Vargas, Louissaint, Colonna, Zaidi, Garcia-Verdugo, Doetsch: "A specialized vascular niche for adult neural stem cells." in: Cell stem cell, Vol. 3, Issue 3, pp. 279-88, (2008) (PubMed).

Freestone, Marker, Grace, Tomlinson, Cunha, Harnden, Thomson: "Sonic hedgehog regulates prostatic growth and epithelial differentiation." in: Developmental biology, Vol. 264, Issue 2, pp. 352-62, (2003) (PubMed).

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: BRDU (BrdU Products)

Target Type: Chemical

Background: Bromodeoxyuridine is a synthetic nucleoside that is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues. It can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical.