TUBA1A antibody

Catalog No. ABIN2856707

Product Details anti-TUBA1A Antibody

Target: TUBA1A (Tuba1a) (Tubulin, alpha 1a (Tuba1a))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TUBA1A antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Cross-Reactivity: Drosophila melanogaster, Human, Mouse, Rat

Characteristics: Rabbit Polyclonal antibody to alpha Tubulin 1A (tubulin, alpha 1a)
alpha Tubulin 1A antibody

Purification: Purified by antigen-affinity chromatography.

Immunogen: Recombinant protein encompassing a sequence within the center region of human alpha Tubulin 1A. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

Comment:

Positive Control: NIH-3T3 , mouse brain , Jurkat , Raji , 293T , A431 , HeLa , HepG2 , H1299 , HCT116 , MCF-7 , NT2D1 , PC-3 , U87-MG , rat brain

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.73 mg/mL

Buffer: 1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-TUBA1A antibody (ABIN2856707)

Chiu, Wen, Wang, Hsu, Tsai, Hung, Tseng, Shyr: "Role of estrogen receptors and Src signaling in mechanisms of bone metastasis by estrogen receptor positive breast cancers." in: Journal of translational medicine, Vol. 15, Issue 1, pp. 97, (2018) (PubMed).

Target Details for TUBA1A

Target: TUBA1A (Tuba1a) (Tubulin, alpha 1a (Tuba1a))

Alternative Name: tubulin alpha 1a (Tuba1a Products)

Background: Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q.

Molecular Weight: 50 kDa

Gene ID: 7846

UniProt: Q71U36

Pathways: Microtubule Dynamics, M Phase