KAT2B antibody (C-Term)

Catalog No. ABIN2856697

Product Details anti-KAT2B Antibody

Target: KAT2B (K(lysine) Acetyltransferase 2B (KAT2B))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KAT2B antibody is un-conjugated

Application: Western Blotting (WB)

Cross-Reactivity: Zebrafish (Danio rerio)

Cross-Reactivity (Details): Zebrafish (88 %)

Characteristics: Rabbit Polyclonal antibody to PCAF (K(lysine) acetyltransferase 2B)
PCAF antibody [C3], C-term

Purification: Purified by antigen-affinity chromatography.

Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human PCAF. The exact sequence is proprietary.

Isotype: IgG

Application Details

Application Notes: Suggested dilution Reference Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceWestern blot1:500-1:3000*

Comment:

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , mouse brain , *MFB

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

References for anti-KAT2B antibody (ABIN2856697)

Sengupta, Yang, Hegde, Hegde, Mitra, Pandey, Dutta, Datarwala, Bhakat, Mitra: "Acetylation of oxidized base repair-initiating NEIL1 DNA glycosylase required for chromatin-bound repair complex formation in the human genome increases cellular resistance to oxidative stress." in: DNA repair, Vol. 66-67, pp. 1-10, (2018) (PubMed).

Cazzalini, Sommatis, Tillhon, Dutto, Bachi, Rapp, Nardo, Scovassi, Necchi, Cardoso, Stivala, Prosperi: "CBP and p300 acetylate PCNA to link its degradation with nucleotide excision repair synthesis." in: Nucleic acids research, Vol. 42, Issue 13, pp. 8433-48, (2014) (PubMed).

Target Details for KAT2B

Target: KAT2B (K(lysine) Acetyltransferase 2B (KAT2B))

Alternative Name: PCAF (KAT2B Products)

Background: CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation.

Cellular Localization: Nucleus

Molecular Weight: 93 kDa

Gene ID: 8850

Pathways: p53 Signaling, Regulation of Carbohydrate Metabolic Process